一种由菁染料混合组装而成的混合聚集型 FRET 探针，用于高度特异性监测线粒体自噬。
A hybrid aggregate FRET probe from the mixed assembly of cyanine dyes for highly specific monitoring of mitochondria autophagy.
机构信息
Beijing National Laboratory for Molecular Sciences (BNLMS), State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry Chinese Academy of Sciences, Beijing, 100190, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR China.
Center for Physicochemical Analysis and Measurement, Institute of Chemistry Chinese Academy of Sciences, Beijing, 100190, PR China.
出版信息
Anal Chim Acta. 2021 Jun 22;1165:338561. doi: 10.1016/j.aca.2021.338561. Epub 2021 Apr 23.
Mitochondria autophagy, also known as mitophagy, is a process in which mitochondria are wrapped by autophagosomes and fused with lysosomes for degradation. This process is essential for mitochondrial quality control. Here, we developed a hybrid aggregate FRET probe through mixed assembly of two cyanine dyes FMOTY and AMTC. In live cells, FMOTY and AMTC exist independently in lysosomes and mitochondria and will not produce interfering FRET background signals. The FRET signal is only generated when mitochondria is transported to lysosomes during mitophagy. This allows the hybridized aggregate to be used as a highly specific probe for monitoring mitophagy.
线粒体自噬，也称为线粒体自噬，是一种线粒体被自噬体包裹并与溶酶体融合进行降解的过程。这个过程对于线粒体的质量控制是至关重要的。在这里，我们通过混合两种花菁染料 FMOTY 和 AMTC 开发了一种杂交聚集 FRET 探针。在活细胞中，FMOTY 和 AMTC 分别独立存在于溶酶体和线粒体中，不会产生干扰的 FRET 背景信号。只有在线粒体自噬过程中线粒体被转运到溶酶体时才会产生 FRET 信号。这使得杂交聚集物可以用作监测线粒体自噬的高度特异性探针。
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