Department of Chemistry, Technology and Biotechnology of Food, Faculty of Chemistry, Gdansk University of Technology.
Department of Obstetrics, Medical University of Gdansk, Gdansk.
J Pediatr Gastroenterol Nutr. 2021 Jun 1;72(6):900-905. doi: 10.1097/MPG.0000000000003071.
The objective of this study was to determine the level of brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) in human milk in the first 2 weeks of breast-feeding and compare the effects of Holder pasteurization (HoP, 62.5 °C, 30 minutes) and microwave pasteurization (MP) at constant temperature (62.5 °C) on the concentraion of both neurotrophic factors (NFs).
Concentration of NFs in human milk was determined using a commercially available ELISA assay.
The average concentration of BDNF and GDNF in milk was 11 ± 6 ng/mL and 336 ± 238 pg/mL, respectively. There was a positive correlation between the concentrations of BDNF and GDNF in human milk and day of lactation (r = 0.441, P < 0.05 and r = 0.482, P < 0.05, respectively). In addition, there was a significant correlation between the levels of BDNF and GDNF (r = 0.366, P < 0.05). HoP and MP for 10 minutes affected both NF levels similarly, causing degradation of BDNF by about 24% and 17%, and GDNF by 47% and 45%, respectively. Use of MP for 5 minutes resulted in preservation of nearly 91% BDNF and 79% GDNF in human milk.
In the pasteurization processes carried out, results showed that GDNF is more susceptible to degradation under the influence of high temperature. To the authors' best knowledge, this is the first study evaluating the effects of HoP and MP at constant temperature on the concentration of NFs in human milk. It was found that the MP for 5 minutes is the optimal method.
本研究旨在确定人乳在母乳喂养头 2 周内脑源性神经营养因子（BDNF）和胶质细胞源性神经营养因子（GDNF）的水平，并比较 Holder 巴氏消毒法（HoP，62.5°C，30 分钟）和微波巴氏消毒法（MP）对两种神经营养因子（NFs）浓度的影响。
使用市售 ELISA 试剂盒测定 NF 浓度。
母乳中 BDNF 和 GDNF 的平均浓度分别为 11±6ng/mL 和 336±238pg/mL。人乳中 BDNF 和 GDNF 的浓度与哺乳期天数呈正相关（r=0.441，P<0.05 和 r=0.482，P<0.05）。此外，BDNF 和 GDNF 水平之间存在显著相关性（r=0.366，P<0.05）。10 分钟的 HoP 和 MP 对两种 NF 水平的影响相似，使 BDNF 降解约 24%和 17%，GDNF 降解 47%和 45%。MP 作用 5 分钟可使母乳中 BDNF 和 GDNF 分别保留近 91%和 79%。
在进行的巴氏消毒过程中，结果表明 GDNF 在高温影响下更容易降解。据作者所知，这是第一项评估 HoP 和 MP 在恒温和对人乳中 NFs 浓度的影响的研究。研究发现，MP 作用 5 分钟是最佳方法。
