Institute of Child Health, Department of Social Pediatrics, İstanbul University, Istanbul, Türkiye.
İstanbul Faculty of Medicine, Department of Medical Biochemistry, İstanbul University, Istanbul, Türkiye.
Eur J Pediatr. 2024 Nov;183(11):4729-4734. doi: 10.1007/s00431-024-05732-y. Epub 2024 Aug 29.
The aim of our study was to show the presence of neurotrophic factors in breast milk that have a significant impact on neurocognitive development of children aged two years and beyond. Mothers expressed at least 5 mL of breast milk into sterile containers when their children 18, 24, and ≥ 25 months of age, and then specimens were transferred to Eppendorf tubes and stored at -20 °C. One day before the analysis, specimens were kept at +4 °C and then thawed at room temperature to prepare them for analysis. Brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF), and S100B neurotrophic growth factor levels were analyzed using the sandwich enzyme-linked immunosorbent assay (ELISA) principles. Sixty-two mothers with children aged 18 months were included in the study. The mean age of the mothers was 33.4 (± 0.71) years. Due to the detection limits of the commercial kits, BDNF and S100B analyses could not be conducted. Therefore, only GDNF was analyzed. The presence of GDNF was found in the breast milk samples taken at 18, 24, and ≥ 25 months, and the median (min max) values were 315,505 ng/mL (193,067 750,718), 316,721 ng/mL (161,278 l-752,252), and 564,577 ng/mL (238,528-781,104) respectively. There were no significant differences between GDNF levels of breast milk samples collected from the same mother at the three different time points (18, 24, and ≥ 25 months) (p = 0.278). Conclusion: Our study was the first to show the presence of neurotrophic factors in the breast milk of mothers with healthy children over one year of age. Our results provide evidence-based data on the importance of breastfeeding until children are at least two years of age. What is Known: • Presence of Brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF), and S100B neurotrophic growth factor have been shown in the breast milk of mothers whose infants are the first year of life. What is New: • Glial Cell Line-Derived Neurotrophic factors continue to present in breast milk of mothers with children aged 18, 24, and ≥ 25 months, without any significant difference in level between months.
我们的研究目的是展示母乳中存在的神经营养因子，这些因子对两岁及以上儿童的神经认知发育有重要影响。当孩子 18、24 和≥25 个月大时，母亲会将至少 5 毫升的母乳挤入无菌容器中，然后将标本转移到 Eppendorf 管中，并储存在-20°C。在分析前一天，标本保存在+4°C，然后在室温下解冻，以准备进行分析。使用夹心酶联免疫吸附测定（ELISA）原理分析脑源性神经营养因子（BDNF）、胶质细胞源性神经营养因子（GDNF）和 S100B 神经营养生长因子水平。该研究纳入了 62 名 18 个月大的儿童的母亲。母亲的平均年龄为 33.4（±0.71）岁。由于商业试剂盒的检测限制，无法进行 BDNF 和 S100B 分析。因此，仅分析了 GDNF。在 18、24 和≥25 个月时采集的母乳样本中均发现了 GDNF，中位数（最小值-最大值）值分别为 315,505ng/ml（193,067-750,718）、316,721ng/ml（161,278-752,252）和 564,577ng/ml（238,528-781,104）。同一母亲在三个不同时间点（18、24 和≥25 个月）采集的母乳样本中 GDNF 水平无显著差异（p=0.278）。结论：我们的研究首次表明，在一岁以上健康儿童的母亲的母乳中存在神经营养因子。我们的研究结果提供了基于证据的数据，证明母乳喂养至儿童至少两岁是非常重要的。已知：·在婴儿一岁以下的母亲的母乳中已发现脑源性神经营养因子（BDNF）、胶质细胞源性神经营养因子（GDNF）和 S100B 神经营养生长因子。新发现：·在 18、24 和≥25 个月大的儿童的母亲的母乳中，胶质细胞源性神经营养因子继续存在，且各月之间的水平没有显著差异。
