Estevez María Belén, Casaux María Laura, Fraga Martín, Faccio Ricardo, Alborés Silvana
Área de Microbiología, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, Uruguay.
Posgrado en Química, Facultad de Química, Universidad de la República, Montevideo, Uruguay.
Front Bioeng Biotechnol. 2021 Apr 26;9:644014. doi: 10.3389/fbioe.2021.644014. eCollection 2021.
Infectious diseases are one of the most important health problems worldwide, one of the main causes being the development of multi-resistant microorganisms. Likewise, the zoonotic potential of some pathogens and their ability to transfer resistance mechanisms, reduce the therapeutic options in both humans and animals. is an important pathogen that affects a wide range of animal species and humans, being Typhimurium one of the most frequent serotypes affecting cattle, causing enteritis, diarrhea, and septicemia. The search for alternative therapeutic approaches has gained importance since the emergence of multidrug resistance to antibiotics and periodic outbreaks of salmonellosis. In this sense, the discovery of new drugs and the development of new strategies, such as the use of nanoparticles with antimicrobial activity, are very promising. The aim of this work was the extracellular production of biogenic silver nanoparticles using fungal extracts and the evaluation of their antimicrobial activity against resistant and multi-resistant Typhimurium strains. We here demonstrated the potential of the biogenic nanoparticles as effective bacteriostatic and bactericidal agents for use in biomedical applications. In addition, Confocal Raman Microscopy and Atomic Force Microscopy were used to advance the understanding of the antimicrobial mechanism of biogenic nanoparticles against these pathogenic strains, the results of which suggested that the nanoparticles produced damage in several bacterial cell structures.
传染病是全球最重要的健康问题之一，主要原因之一是多重耐药微生物的出现。同样，一些病原体的人畜共患病潜力及其转移耐药机制的能力，减少了人类和动物的治疗选择。鼠伤寒沙门氏菌是一种重要的病原体，可感染多种动物和人类，是影响牛的最常见血清型之一，可引起肠炎、腹泻和败血症。自从出现对抗生素的多重耐药性以及沙门氏菌病的周期性爆发以来，寻找替代治疗方法变得越来越重要。从这个意义上说，发现新药和开发新策略，如使用具有抗菌活性的纳米颗粒，非常有前景。这项工作的目的是利用真菌提取物在细胞外生产生物银纳米颗粒，并评估其对耐药和多重耐药鼠伤寒沙门氏菌菌株的抗菌活性。我们在此证明了生物纳米颗粒作为有效的抑菌和杀菌试剂用于生物医学应用的潜力。此外，共聚焦拉曼显微镜和原子力显微镜被用于进一步了解生物纳米颗粒对这些致病菌株的抗菌机制，其结果表明纳米颗粒对几种细菌细胞结构造成了损伤。
