Zhang Hongjia, Wu Tao, Li Zhao, Huang Kai, Kim Na-Eun, Ma Ziming, Kwon Soon-Wook, Jiang Wenzhu, Du Xinglin
Jilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant Science, Jilin University, No. 5333 Xi'an Road, Changchun, 130062, China.
Department of Plant Bioscience, College of Natural Resources and Life Science, Pusan National University, Milyang, 50463, Republic of Korea.
Rice (N Y). 2021 May 12;14(1):42. doi: 10.1186/s12284-021-00485-w.
Cold stress is the main abiotic stress in rice, which seriously affects the growth and yield of rice. Identification of cold tolerance genes is of great significance for rice to solve these problems. GATA-family transcription factors involve diverse biological functions, however, their role in cold tolerance in rice remains unclear.
In this study, a GATA-type zinc finger transcription factor OsGATA16, which can improve cold tolerance, was isolated and characterized from rice. OsGATA16 belongs to OsGATA subfamily-II and contains 11 putative phosphorylation sites, a nuclear localization signal (NLS), and other several conserved domains. OsGATA16 was expressed in all plant tissues, with the strongest in panicles. It was induced by cold and ABA treatments, but was repressed by drought, cytokinin and JA, and acted as a transcriptional suppressor in the nucleus. Overexpression of OsGATA16 improves cold tolerance of rice at seedling stage. Under cold stress treatments, the transcription of four cold-related genes OsWRKY45-1, OsSRFP1, OsCYL4, and OsMYB30 was repressed in OsGATA16-overexpressing (OE) rice compared with wild-type (WT). Interestingly, OsGATA16 bound to the promoter of OsWRKY45-1 and repressed its expression. In addition, haplotype analysis showed that OsGATA16 polarized between the two major rice subspecies japonica and indica, and had a non-synonymous SNP8 (336) associated with cold tolerance.
OsGATA16 is a GATA transcription factor, which improves cold tolerance at seedling stage in rice. It acts as a positive regulator of cold tolerance by repressing some cold-related genes such as OsWRKY45-1, OsSRFP1, OsCYL4 and OsMYB30. Additionally, OsGATA16 has a non-synonymous SNP8 (336) associated with cold tolerance on CDS region. This study provides a theoretical basis for elucidating the mechanism of cold tolerance in rice and new germplasm resources for rice breeding.
冷胁迫是水稻主要的非生物胁迫，严重影响水稻的生长和产量。鉴定耐寒基因对水稻解决这些问题具有重要意义。GATA家族转录因子具有多种生物学功能，然而，它们在水稻耐寒性中的作用尚不清楚。
本研究从水稻中分离并鉴定了一个能提高耐寒性的GATA型锌指转录因子OsGATA16。OsGATA16属于OsGATA亚家族II，包含11个假定的磷酸化位点、一个核定位信号（NLS）和其他几个保守结构域。OsGATA16在所有植物组织中均有表达，在穗中表达最强。它受冷和脱落酸处理诱导，但受干旱、细胞分裂素和茉莉酸抑制，并在细胞核中作为转录抑制因子发挥作用。OsGATA16的过表达提高了水稻苗期的耐寒性。在冷胁迫处理下，与野生型（WT）相比，OsGATA16过表达（OE）水稻中四个与冷相关的基因OsWRKY45-1、OsSRFP1、OsCYL4和OsMYB30的转录受到抑制。有趣的是，OsGATA16与OsWRKY45-1的启动子结合并抑制其表达。此外，单倍型分析表明，OsGATA16在两个主要水稻亚种粳稻和籼稻之间存在分化，并且有一个与耐寒性相关的非同义SNP8（336）。
OsGATA16是一个GATA转录因子，可提高水稻苗期的耐寒性。它通过抑制一些与冷相关的基因如OsWRKY45-1、OsSRFP1、OsCYL4和OsMYB30，作为耐寒性的正调控因子发挥作用。此外，OsGATA16在CDS区域有一个与耐寒性相关的非同义SNP8（336）。本研究为阐明水稻耐寒机制提供了理论依据，并为水稻育种提供了新的种质资源。
