Sun Chen-Chen, Su Jie, Yan Mei-Qiu, Chen Qing-Qing, Chen Su-Hong, Lyu Gui-Yuan
Zhejiang Chinese Medical University Hangzhou 310053, China.
Zhejiang University of Technology Hangzhou 310014, China.
Zhongguo Zhong Yao Za Zhi. 2021 Apr;46(7):1674-1680. doi: 10.19540/j.cnki.cjcmm.20200104.402.
Dendrobium officinale is a sacred product for nourishing Yin and has a clear "thick gastrointestinal" effect. Modern pharmacological studies had found that it could improve gastrointestinal function. This study observed the improvement effect of D. officinale on constipation model mice with Yin deficiency caused by warm-drying medicine. It provided experimental basis for the treatment of Yin deficiency constipation. The male and female ICR mice were randomly divided into normal group, model group, D. officinale high, medium and low dose groups(0.6, 0.4, 0.2 g·kg~(-1)), and phenolphthalein tablets group. The model mice of Yin deficiency constipation were established by gavage with warm-drying medicine. The overall state and body temperature of the mice were observed and recorded. The number of feces, feces weight, fecal moisture content and intestinal propulsion were measured. The morphological damage of colon tissue was observed by hematoxylin-eosin(HE) staining. The expression of inducible nitric oxide synthase(iNOS) in the colon was detected by Western blot and immunohistochemical method. The expression of iNOS mRNA in the colon was detected by Real-time fluorescence quantitative PCR, and the serum cyclic guanosine phosphate(cGMP) level was detected the enzyme-linked immunosorbent assay(ELISA). The results showed that D. candidum could reduce the body temperature of mice with Yin deficiency constipation, increase the number of feces, wet feces, dry feces and intestinal propulsion ability, reduce the expression of iNOS protein and mRNA in the colon, and reduce the content of cGMP in the serum. It showed that D. candidum could improve the symptoms of Yin deficiency constipation mice caused by warm-drying medicine, and the mechanism may be related to reducing the expression of iNOS in the colon and increasing intestinal motility.
铁皮石斛是滋阴的圣品，具有明确的“厚肠胃”功效。现代药理研究发现其能改善胃肠功能。本研究观察了铁皮石斛对温燥药致阴虚便秘模型小鼠的改善作用，为阴虚便秘的治疗提供实验依据。将雌雄ICR小鼠随机分为正常组、模型组、铁皮石斛高、中、低剂量组（0.6、0.4、0.2 g·kg⁻¹）和酚酞片组。采用温燥药灌胃法建立阴虚便秘模型小鼠。观察并记录小鼠的整体状态和体温。测量小鼠的粪便粒数、粪便重量、粪便含水量及肠推进率。采用苏木精-伊红（HE）染色观察结肠组织的形态损伤。采用蛋白质免疫印迹法和免疫组织化学法检测结肠组织中诱导型一氧化氮合酶（iNOS）的表达。采用实时荧光定量PCR检测结肠组织中iNOS mRNA的表达，采用酶联免疫吸附测定（ELISA）法检测血清环磷酸鸟苷（cGMP）水平。结果显示，铁皮石斛可降低阴虚便秘小鼠的体温，增加粪便粒数、湿粪、干粪及肠推进能力，降低结肠组织中iNOS蛋白和mRNA的表达，降低血清中cGMP含量。表明铁皮石斛可改善温燥药致阴虚便秘小鼠的症状，其机制可能与降低结肠组织中iNOS的表达、增强肠道蠕动有关。
