[木榄内生真菌枝孢霉JJM22的次生代谢产物研究]
[Study on secondary metabolites of endophytic fungus Cladosporium sp. JJM22 hosted in Ceriops tagal].
作者信息
Li Zhen, Yang Jing-Yu, Caj Jin, Ouyang Zi-Juan, Zhou Chu-Han, Chen Guang-Ying, Zhou Xue-Ming
机构信息
Key Laboratory of Tropical Medicinal Resource Chemistry of Ministry of Education, Key Laboratory of Tropical Medicinal Plant Chemistry of Hainan Province, Hainan Normal University Haikou 571158, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2021 Apr;46(8):2079-2083. doi: 10.19540/j.cnki.cjcmm.20201223.601.
Nine secondary metabolites(S)-5-hydroxy-4-methylchroman-2-one(1), 4-methoxynaphthalene-1,5-diol(2), 8-methoxynaphthalene-1,7-diol(3), 1,8-dimethoxynaphthalene(4),(2R,4S)-2,3-dihydro-2-methyl-benzopyran-4,5-diol(5),(2R,4R)-3,4-dihydro-4-methoxy-2-methyl-2H-1-benzopyran-5-ol(6), 7-O-α-D-ribosyl-2,3-dihydro-5-hydroxy-2-methyl-chromen-4-one(7),(R)-3-methoxyl-1-(2,6-dihydroxyphenyl)-butan-1-one(8) and helicascolide A(9) were isolated from endophytic fungus Cladosporium sp. JJM22 by using column chromatographies of silica gel and ODS, and semi-preparative HPLC. Their structures were analyzed on the basis of spectroscopic and chemical data, especially NMR and MS. All isolated compounds were evaluated for their anti-inflammatory activities by examining the inhibitory activities on nitric oxide(NO) production induced by lipopolysaccharide in mouse macrophage RAW264.7 cells in vitro. Compounds 2-4 showed inhibitory activities.
通过硅胶柱色谱、ODS柱色谱和半制备型高效液相色谱法，从内生真菌枝孢菌属JJM22中分离出9种次生代谢产物：(S)-5-羟基-4-甲基色满-2-酮(1)、4-甲氧基萘-1,5-二醇(2)、8-甲氧基萘-1,7-二醇(3)、1,8-二甲氧基萘(4)、(2R,4S)-2,3-二氢-2-甲基苯并吡喃-4,5-二醇(5)、(2R,4R)-3,4-二氢-4-甲氧基-2-甲基-2H-1-苯并吡喃-5-醇(6)、7-O-α-D-核糖基-2,3-二氢-5-羟基-2-甲基色烯-4-酮(7)、(R)-3-甲氧基-1-(2,6-二羟基苯基)丁-1-酮(8)和螺旋灰包菌素A(9)。基于光谱和化学数据，尤其是核磁共振和质谱，对它们的结构进行了分析。通过检测体外小鼠巨噬细胞RAW264.7中脂多糖诱导的一氧化氮(NO)产生的抑制活性，对所有分离出的化合物的抗炎活性进行了评估。化合物2-4表现出抑制活性。
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