[来自宿主于桐花树的内生真菌赫克青霉JX4的两种新的松脂醇衍生物]
[Two new pinophol derivatives from endophytic fungus Penicillium herquei JX4 hosted in Ceriops tagal].
作者信息
Lao Xian-Lei, Wu Mei-Zhu, Zheng Ting, Chen Guang-Ying, Zheng Cai-Juan, Chen Ya-Lin, Zhou Jin-Qi, Feng Shuo, Zhou Xue-Ming, Song Xin-Ming
机构信息
Key Laboratory of Tropical Medicinal Resource Chemistry of Ministry of Education, Key Laboratory of Tropical Medicinal Plant Chemistry of Hainan Province, Hainan Normal University Haikou 571158, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2024 Jul;49(13):3548-3551. doi: 10.19540/j.cnki.cjcmm.20240405.202.
An OSMAC strategy was used to study secondary metabolites and anti-inflammatory activities of the endophytic fungus Penicillium herquei JX4 hosted in Ceriops tagal. The PDB ferment of fungus P. herquei JX4 was isolated, purified, and identified by using silica gel column chromatography, gel column chromatography, octadecylsilyl(ODS) column chromatography, and semi-preparative high-performance liquid chromatography. Two new pinophol derivatives, pinophol H(1) and pinophol I(2) were isolated and identified, and they were evaluated in terms of the inhibitory activities against the nitric oxide(NO) production induced by lipopolysaccharide(LPS) in mouse macrophage RAW264.7 cells. The results showed that compound 1 had significant inhibitory activity on NO production, with an IC_(50) value of 8.12 μmol·L~(-1).
采用OSMAC策略研究了桐花树内生真菌黑曲霉JX4的次生代谢产物和抗炎活性。通过硅胶柱色谱、凝胶柱色谱、十八烷基硅烷（ODS）柱色谱和半制备高效液相色谱对黑曲霉JX4的PDB发酵产物进行分离、纯化和鉴定。分离鉴定了两种新的松脂醇衍生物，松脂醇H（1）和松脂醇I（2），并对它们在小鼠巨噬细胞RAW264.7细胞中对脂多糖（LPS）诱导的一氧化氮（NO）产生的抑制活性进行了评价。结果表明，化合物1对NO产生具有显著的抑制活性，IC50值为8.12μmol·L-1。
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