Griffiths R P, Hayasaka S S, McNamara T M, Morita R Y
Appl Environ Microbiol. 1977 Dec;34(6):801-5. doi: 10.1128/aem.34.6.801-805.1977.
Two methods for determining relative microbial activity in the marine environment were compared. In one method, a single concentration of a labeled substrate was used to calculate rates of substrate utilization; in the other, multiple concentrations of the same substrate (heterotrophic activity method) were used to calculate maximum potential substrate utilization rates. These studies were made on 232 seawater and 79 sediment samples taken from a variety of marine environments. The highest correlations between these two methods were seen in the sediment samples tested. The lowest correlation coerfficient seen in the sediment samples was 0.90, and the highest was 0.98. In seawater samples (six studies), the lowest correlation coefficient was 0.77 and the highest was 0.95. The correlation between these two methods was also substrate concentration dependent. Higher correlation coefficients were observed when higher substrate concentrations were used. Under certain conditions, these two methods appear to be comparable for estimating relative levels of microbial activity in the marine environment.
对两种测定海洋环境中相对微生物活性的方法进行了比较。一种方法是使用单一浓度的标记底物来计算底物利用率；另一种方法是使用相同底物的多种浓度（异养活性法）来计算最大潜在底物利用率。这些研究是对从各种海洋环境中采集的232个海水样本和79个沉积物样本进行的。在测试的沉积物样本中，这两种方法之间的相关性最高。在沉积物样本中看到的最低相关系数为0.90，最高为0.98。在海水样本（六项研究）中，最低相关系数为0.77，最高为0.95。这两种方法之间的相关性也取决于底物浓度。当使用较高底物浓度时，观察到较高的相关系数。在某些条件下，这两种方法在估计海洋环境中微生物活性的相对水平方面似乎具有可比性。
