Jaffar Farah Hanan Fathihah, Osman Khairul, Hui Chua Kien, Zulkefli Aini Farzana, Ibrahim Siti Fatimah
Department of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia (UKM), Cheras, Kuala Lumpur, Malaysia.
Forensic Science Program, Faculty of Health Sciences, Universiti Kebangsaan Malaysia (UKM), Bangi, Selangor, Malaysia.
Front Pharmacol. 2021 Mar 30;12:631402. doi: 10.3389/fphar.2021.631402. eCollection 2021.
Edible bird's nest (EBN) is reported to have a positive proliferative effect and contain male reproductive hormones. Spermatogonia cells proliferate during spermatogenesis under male reproductive hormones stimulation that include testosterone, follicle-stimulating hormone (FSH), and luteinizing hormone (LH). Characterization of EBN through liquid chromatography-mass spectrometry (LCMS) has found testosterone as a base peak. Six types of amino acids, estradiol and sialic acid were among the major peaks that have been characterized. Based on the presence of these reproductive components, this study evaluated different doses of EBN on sperm parameters and male reproductive hormones of Sprague Dawley rats. Sixteen Sprague Dawley rats at the age of eight weeks were randomly and equally divided into four groups, which are Control, 10 mg/kg BW/d 50 mg/kg BW/d, and 250 mg/kg BW/d EBN group. The rats were fed with EBN enriched pellet daily and water ad-libitum. Rats were sacrificed and the organ was weighed for organ coefficients after eight weeks of treatment. Sperm concentration, percentage of sperm motility, and sperm viability were evaluated. Meanwhile, ELISA method was used to measure testosterone, FSH, and LH. Findings showed that there were no significant differences in organ coefficient between groups. Supplementation of 250 mg/kg BW/d EBN demonstrated a significant increase in sperm concentration, percentage of sperm motility as well as FSH and LH level compared to 10 mg/kg BW/d group. There was a dose-dependent increase in testosterone level but was not significant between groups. Based on these findings, EBN is concluded to have crucial effects on male reproductive parameters.
据报道，食用燕窝（EBN）具有积极的增殖作用，并含有雄性生殖激素。精原细胞在包括睾酮、促卵泡激素（FSH）和促黄体生成素（LH）在内的雄性生殖激素刺激下，在精子发生过程中增殖。通过液相色谱-质谱联用（LCMS）对EBN进行表征，发现睾酮为基峰。六种氨基酸、雌二醇和唾液酸是已鉴定的主要峰之一。基于这些生殖成分的存在，本研究评估了不同剂量的EBN对斯普拉格-道利大鼠精子参数和雄性生殖激素的影响。16只8周龄的斯普拉格-道利大鼠被随机且平均分为四组，即对照组、10mg/kg体重/天、50mg/kg体重/天和250mg/kg体重/天的EBN组。大鼠每天喂食富含EBN的颗粒饲料，并自由饮水。治疗8周后，处死大鼠并称重器官以计算器官系数。评估精子浓度、精子活力百分比和精子存活率。同时，采用酶联免疫吸附测定（ELISA）法测定睾酮、FSH和LH。结果表明，各组之间的器官系数无显著差异。与10mg/kg体重/天组相比，补充250mg/kg体重/天的EBN可显著提高精子浓度、精子活力百分比以及FSH和LH水平。睾酮水平呈剂量依赖性升高，但各组之间无显著差异。基于这些发现，得出结论：EBN对雄性生殖参数具有关键作用。
