Jiangxi Province Key Laboratory of Animal Nutrition, Jiangxi Agricultural University, Nanchang 330045, Jiangxi, China.
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100094, China.
Food Res Int. 2021 May;143:110270. doi: 10.1016/j.foodres.2021.110270. Epub 2021 Mar 9.
Curcumin (Cur) is a natural polyphenol with beneficial effect against obesity and related metabolic disorders, but its precise mechanisms of action remain to be defined due to its limited systemic bioavailability. We hypothesized that gut microbiota may be a prospective therapeutic target for Cur-induced metabolic benefits. This study aimed to investigate whether the metabolic adaptations resulting from Cur supplementation were mediated by the gut microbiota in high-fat diet (HFD)-fed obese mice. C57BL/6 mice were fed a control diet or a HFD diet with or without 0.2% Cur for 10 weeks. Lipid profiles, insulin sensitivity, hepatic metabolism, gut microbiota composition and short-chain fatty acid (SCFA) production were determined. Dietary Cur reduced fat mass, hepatic steatosis and circulating lipopolysaccharide levels and improved the insulin sensitivity in HFD-fed mice. More importantly, Cur supplementation modulated the gut microbiota composition and ameliorated intestinal dysbiosis by decreasing the ratio of Firmicutes/Bacteroidetes and endotoxin-producing Desulfovibrio bacteria and increasing the abundance of Akkermansia population and SCFA-producing bacteria, such as Bacteroides, Parabacteroides, Alistipes and Alloprevotella, along with increases in caecal and colonic SCFA concentrations. These dominant bacterial genera altered by Cur showed strong correlations with the obesity-related metabolic parameters in HFD-fed mice. In conclusion, our data suggest that Cur alleviated metabolic features of hepatic steatosis and insulin resistance in HFD-fed obese mice, which might be associated with the modulation of gut microbiota composition and metabolites.
姜黄素（Cur）是一种天然多酚，对肥胖和相关代谢紊乱具有有益作用，但由于其系统生物利用度有限，其确切的作用机制仍有待确定。我们假设肠道微生物群可能是姜黄素诱导代谢益处的一个有前景的治疗靶点。本研究旨在探讨姜黄素补充后产生的代谢适应是否通过高脂肪饮食（HFD）喂养肥胖小鼠的肠道微生物群介导。将 C57BL/6 小鼠喂食对照饮食或 HFD 饮食，或在 HFD 饮食中添加 0.2%的姜黄素，持续 10 周。测定脂质谱、胰岛素敏感性、肝代谢、肠道微生物群组成和短链脂肪酸（SCFA）产生。饮食姜黄素可减少肥胖小鼠的脂肪量、肝脂肪变性和循环脂多糖水平，并改善胰岛素敏感性。更重要的是，姜黄素补充通过降低厚壁菌门/拟杆菌门的比例和产内毒素的脱硫弧菌来调节肠道微生物群组成，并改善肠道失调，增加阿克曼氏菌属和产 SCFA 的细菌的丰度，如拟杆菌属、副拟杆菌属、双歧杆菌属和 Alloprevotella 属，同时增加盲肠和结肠 SCFA 浓度。这些被姜黄素改变的主要细菌属与 HFD 喂养肥胖小鼠的肥胖相关代谢参数具有很强的相关性。总之，我们的数据表明，姜黄素减轻了 HFD 喂养肥胖小鼠的肝脂肪变性和胰岛素抵抗的代谢特征，这可能与肠道微生物群组成和代谢物的调节有关。
