巴西绿蜂胶醇溶液对颌面修复聚合物中金黄色葡萄球菌生物膜的消毒效果。
Disinfectant effects of Brazilian green propolis alcohol solutions on the Staphylococcus aureus biofilm of maxillofacial prosthesis polymers.
机构信息
Predoctoral student, Department of Oral Surgery, Pathology, and Clinical Dentistry, School of Dentistry, Federal University of Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil.
Graduate student, Department of Oral Surgery, Pathology, and Clinical Dentistry, School of Dentistry, Federal University of Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil.
出版信息
J Prosthet Dent. 2022 Dec;128(6):1405-1411. doi: 10.1016/j.prosdent.2021.03.025. Epub 2021 May 13.
STATEMENT OF PROBLEM
Brazilian green propolis may be an alternative product that reduces the development of a microbial biofilm on the polymers used for maxillofacial prostheses. However, its effects as a disinfectant have not been fully established.
PURPOSE
The purpose of this in vitro study was to investigate the effects of Brazilian green propolis alcohol solutions against the Staphylococcus aureus biofilm on polymers used in maxillofacial prostheses, the maxillofacial silicone elastomer (MDX4-4210), and specific acrylic resins for ocular prostheses.
MATERIAL AND METHODS
A total of 324 disk-shaped specimens (3×10 mm) of each material were fabricated. All specimens were contaminated with S. aureus (10 cells/mL) to assess the antibiofilm activity of immersion solutions and protocols. Thus, 162 specimens of each material were randomly distributed and equally divided into 5 groups of disinfectants and 1 control group: 3 separate groups of 2.5%, 5%, and 10% propolis alcohol solutions, 1 group of 5% propolis alcohol gel, a positive control group of 2% chlorhexidine gluconate, and a negative control group of distilled water. Specimens (n=9) were disinfected by immersion for 5, 10, and 15 minutes and immersed in culture medium for 24 hours. Any notable turgescence in the final medium was considered indicative of a biofilm. The effects of disinfectants were analyzed by a turbidity assay and by scanning electron microscopy. Data were analyzed descriptively.
RESULTS
The final medium with specimens disinfected with 10% propolis alcohol solution showed no turbidity, indicating constant efficacy against the S. aureus biofilm. Similarly, these findings were observed in the 2% chlorhexidine gluconate group. Scanning electron microscopy images demonstrated that the surface of the polymers treated with 10% propolis alcohol solution did not show bacterial colonies.
CONCLUSIONS
Disinfection with 10% green propolis alcohol solution was effective in eliminating the S. aureus biofilm from specimens of maxillofacial elastomer and N1 acrylic resin specific to ocular prostheses by immersion for 5 minutes.
问题陈述
巴西绿蜂胶可能是一种替代产品，可以减少用于颌面修复体的聚合物上微生物生物膜的形成。然而，其作为消毒剂的效果尚未完全确定。
目的
本体外研究的目的是调查巴西绿蜂胶醇溶液对颌面修复体用聚合物、颌面硅橡胶弹性体（MDX4-4210）和特定的眼部假肢用丙烯酸树脂上金黄色葡萄球菌生物膜的影响。
材料和方法
共制作了 324 个各材料的圆盘状试件（3×10mm）。所有试件均用金黄色葡萄球菌（10 个细胞/mL）污染，以评估浸泡溶液和方案的抗生物膜活性。因此，每个材料的 162 个试件被随机分配并平均分为 5 组消毒剂和 1 个对照组：3 组 2.5%、5%和 10%的蜂胶醇溶液，1 组 5%的蜂胶醇凝胶，1 组 2%葡萄糖酸氯己定阳性对照组和 1 组蒸馏水阴性对照组。试件（n=9）用浸泡法消毒 5、10 和 15 分钟，然后在培养介质中浸泡 24 小时。最后培养基中任何明显的肿胀都被认为是生物膜的指示。消毒剂的效果通过浊度测定法和扫描电子显微镜进行分析。数据进行描述性分析。
结果
用 10%蜂胶醇溶液消毒的最终培养基无浑浊，表明对金黄色葡萄球菌生物膜持续有效。同样，在 2%葡萄糖酸氯己定组也观察到了这一结果。扫描电子显微镜图像显示，用 10%蜂胶醇溶液处理的聚合物表面没有细菌菌落。
结论
通过浸泡 5 分钟，10%绿蜂胶醇溶液对颌面弹性体和特定于眼部假肢的 N1 丙烯酸树脂标本中的金黄色葡萄球菌生物膜进行消毒是有效的。
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