Almeida Alda Graciele Claudio Dos Santos, Bruna Camila Quartim de Moraes, Moriya Giovana Abrahão de Araújo, Navarini Alessandra, Sasagawa Suzethe Matiko, Mimica Lycia M J, Gambale Valderez, Graziano Kazuko Uchikawa
Nursing School of Universidade Federal de Alagoas, Arapiraca, Brazil.
Universidade de São Paulo, São Paulo, Brazil.
J Occup Health. 2021 Jan;63(1):e12234. doi: 10.1002/1348-9585.12234.
Guidelines recommend that the cleaning area in a Central Sterile Supply Department (CSSD) maintain a negative pressure of the environmental air, but how much this system can impact the contamination of the air by bioaerosols in the area is not known. The objective of this study was to assess the impact of negative pressure on CSSD by evaluating the microbiological air quality of this sector.
Microbiological air samples were collected in two CSSD in the same hospital: one with and one without a negative air pressure system. Outdoor air samples were collected as a comparative control. Andersen six-stage air sampler was used to obtain the microbiological air samples.
The concentration of bioaerosols in the CSSD without negative pressure was 273.15 and 206.71 CFU/m , while in the CSSD with negative pressure the concentration of bioaerosols was 116.96 CFU/m and 131.10 CFU/m . The number of isolated colonies in the negative pressure CSSD was significantly lower (P = .01541).
The findings showed that the negative pressure system in the CSSD cleaning area contributed to the quantitative reduction in bioaerosols. However, the concentration of bioaerosols was lower than that established in the guideline for indoor air quality of many countries. Therefore, it cannot be concluded that CSSDs which do not have a negative pressure system in their cleaning area offer occupational risk.
指南建议中央消毒供应室（CSSD）的清洁区域保持环境空气负压，但该系统对该区域生物气溶胶污染空气的影响程度尚不清楚。本研究的目的是通过评估该部门的微生物空气质量来评估负压对CSSD的影响。
在同一家医院的两个CSSD中采集微生物空气样本：一个有负压系统，一个没有负压系统。采集室外空气样本作为对照。使用安德森六级空气采样器获取微生物空气样本。
无负压的CSSD中生物气溶胶浓度分别为273.15和206.71 CFU/m³，而有负压的CSSD中生物气溶胶浓度为116.96 CFU/m³和131.10 CFU/m³。负压CSSD中分离菌落数显著更低（P = 0.01541）。
研究结果表明，CSSD清洁区域的负压系统有助于生物气溶胶的定量减少。然而，生物气溶胶浓度低于许多国家室内空气质量指南规定的浓度。因此，不能得出清洁区域没有负压系统的CSSD存在职业风险的结论。
