Department of environmental health engineering, School of health, Shiraz University of Medical Sciences, Shiraz, Iran.
Research Center for Health Sciences, Institute of health, Shiraz University of Medical Sciences, Shiraz, Iran.
Environ Sci Pollut Res Int. 2019 Jun;26(17):16868-16876. doi: 10.1007/s11356-017-0939-5. Epub 2018 Jan 3.
Fungi are one of the bioaerosols in indoor air of hospitals. They have adverse effects on staff and patients. The aim of this study was to investigate the effects of three incubation temperature on the density and composition of airborne fungi in an indoor and outdoor space of hospital. Sabouraud dextrose agar was used for culture the fungi. For improvement of aseptic properties, chloramphenicol was added to this medium. The density of airborne fungi was less than 282 CFU/m. The highest density was detected in emergency room and the lowest of them was in neonatal intensive care unit (NICU) and operation room (OR). Results showed that fungi levels at 25 °C were higher than 37 and 15 °C (p = 0.006). In addition, ten different genera of fungi were identified in all departments. The predominant fungi were Fusarium spp., Penicillium spp., Paecilomyces spp., and Aspergillus niger. Moreover, the density and trend of distribution of Fusaruim spp. in the indoor space was directivity to outdoor space by ventilation system. The present study has provided that incubation temperature had effect on airborne fungi remarkably. We are suggested that more studies would be conducted on incubation temperature and other ambient factors on airborne fungi.
真菌是医院室内空气中的一种生物气溶胶。它们对工作人员和患者都有不良影响。本研究旨在调查三种孵育温度对医院室内外空气浮游真菌密度和组成的影响。使用沙氏葡萄糖琼脂培养基来培养真菌。为了提高无菌性能，在该培养基中添加了氯霉素。空气中真菌的密度小于 282 CFU/m。在急诊室检测到的真菌密度最高，而在新生儿重症监护病房（NICU）和手术室（OR）中检测到的真菌密度最低。结果表明，25°C 时的真菌水平高于 37°C 和 15°C（p=0.006）。此外，在所有科室都鉴定出了十种不同属的真菌。主要真菌为镰刀菌属、青霉属、拟青霉属和黑曲霉。此外，通风系统使室内空间中镰刀菌属的密度和分布趋势直接指向室外空间。本研究表明，孵育温度对空气浮游真菌有显著影响。我们建议对孵育温度和其他环境因素对空气浮游真菌的影响进行更多的研究。
