Bolda Mariano Luísa Nathália, Boeing Thaise, Cechinel Filho Valdir, Niero Rivaldo, Gasparotto Junior Arquimedes, Mota da Silva Luisa, de Souza Priscila
Programa de Pós-graduação em Ciências Farmacêuticas (PPGCF), Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí (UNIVALI), Rua Uruguai, 458, Centro, 88302-901, Itajaí, Brazil.
Laboratório de Farmacologia Cardiovascular (LaFaC), Faculdade de Ciências da Saúde, Universidade Federal da Grande Dourados, R. João Rosa Góes, 1761, CEP 79825-070, Dourados, Brazil.
Evid Based Complement Alternat Med. 2021 Apr 20;2021:5510053. doi: 10.1155/2021/5510053. eCollection 2021.
The previous study showed that 1,5,8-trihydroxy-4',5'-dimethyl-2H-pyrano(2,3 : 3,2)-4-(3-methylbut-2-enyl) xanthone (TDP) obtained from Rusby (Clusiaceae) branches induces acute diuresis in normotensive (NTR) and spontaneously hypertensive rats (SHR) after 8 h of the experiment. In complementarity, the present study evaluated the prolonged diuretic and renoprotective effects of TDP in both NTR and SHR. The animals received, once a day, oral treatment with TDP (0.1 mg/kg), hydrochlorothiazide (10 mg/kg), or vehicle (VEH; 10 mL/kg). At the end of 7 days, the urine, blood, and kidney samples were collected for biochemical and histological analyzes. The urinary volume of both NTR and SHR after 7 days of treatment with the TDP was significantly increased, associated with augmented urinary electrolyte excretion levels. The treatments did not modify the urinary pH values nor the parameters analyzed in plasma (Na, K, Cl, and Ca). Concerning the renal analyzes, when compared with the VEH-treated NTR group, while the activity of the enzymes catalase (CAT) and N-acetyl--D-glucosaminidase (NAG), as well as nitrite levels, were increased, the generation of lipid hydroperoxides and the activity of the enzyme myeloperoxidase (MPO) were unaltered. On the other hand, the activities of superoxide dismutase (SOD) and glutathione -transferase (GST) and the levels of reduced glutathione (GSH) in kidney homogenates of the SHR group were decreased. However, TDP augmented the levels of GSH and GST activities and reduced the levels of nitrite and the activities of CAT and MPO, when compared with VEH-treated only SHR. Besides, the treatment with TDP alleviated the morphological changes of the renal corpuscle region of SHR. Together, these results revealed the prolonged diuretic effect of TDP and their renal protective effect by improving the antioxidative capacity.
先前的研究表明，从鲁氏藤黄（藤黄科）树枝中提取的1,5,8-三羟基-4',5'-二甲基-2H-吡喃并(2,3 : 3,2)-4-(3-甲基丁-2-烯基)呫吨酮（TDP）在实验8小时后可使正常血压大鼠（NTR）和自发性高血压大鼠（SHR）产生急性利尿作用。作为补充，本研究评估了TDP对NTR和SHR的长期利尿和肾脏保护作用。动物每天接受一次口服TDP（0.1 mg/kg）、氢氯噻嗪（10 mg/kg）或赋形剂（VEH；10 mL/kg）治疗。在7天结束时，收集尿液、血液和肾脏样本进行生化和组织学分析。用TDP治疗7天后，NTR和SHR的尿量均显著增加，同时尿电解质排泄水平升高。这些治疗并未改变尿液pH值或血浆中分析的参数（钠、钾、氯和钙）。关于肾脏分析，与接受VEH治疗的NTR组相比，过氧化氢酶（CAT）和N-乙酰-β-D-氨基葡萄糖苷酶（NAG）的活性以及亚硝酸盐水平升高，而脂质氢过氧化物的生成和髓过氧化物酶（MPO）的活性未改变。另一方面，SHR组肾脏匀浆中超氧化物歧化酶（SOD）和谷胱甘肽-S-转移酶（GST）的活性以及还原型谷胱甘肽（GSH）的水平降低。然而，与仅接受VEH治疗的SHR相比，TDP提高了GSH和GST的活性水平，降低了亚硝酸盐水平以及CAT和MPO的活性。此外，TDP治疗减轻了SHR肾小体区域的形态学变化。总之，这些结果揭示了TDP的长期利尿作用及其通过提高抗氧化能力产生的肾脏保护作用。
