Programa de Pós-graduação em Ciências Farmacêuticas (PPGCF), Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí (UNIVALI), Rua Uruguai, 458, Centro, 88302-901 Itajaí, Brazil.
J Pharm Pharmacol. 2024 Jan 27;76(2):106-114. doi: 10.1093/jpp/rgad123.
The diuretic and kidney protective effect of the 3-demethyl-2-geranyl-4-prenylbellidifoline (DGP) were evaluated in rats.
The normotensive (NTR) and spontaneously hypertensive rats (SHR) received, once a day for 7 days, oral treatment with DGP (0.1 mg/kg), hydrochlorothiazide (10 mg/kg), or vehicle (10 ml/kg). Urine, blood, and kidney samples were collected for further analysis.
The urine and Na+ elimination content were significantly higher in the groups that received DGP. Furthermore, a Ca2+-sparing action was detected in the urine of DGP-treated groups, which was consistent with the reduction in calcium oxalate crystal formation. Relevantly, the treatment did not change the parameters examined in the blood. Concerning the renal analyses, DGP treatment recovered the morphological damages of the kidney corpuscle area of SHR. In addition to the differences observed between the NTR and SHR vehicle groups, DGP augmented the amount of reduced glutathione and the activity of glutathione S-transferase GST while reducing the catalase and N-acetyl-β-D-glucosaminidase activity and nitrite levels.
Together, this study displayed the prolonged diuretic action of DGP and its natriuretic, Ca2+-sparing, and antiurolytic effects. The antioxidative and anti-inflammatory effects of DGP were evidenced in SHR kidneys, opening perspectives for further studies regarding the benefits of this xanthone.
评估 3-去甲基-2-香叶基-4-异戊烯基小檗红碱（DGP）在大鼠体内的利尿和肾脏保护作用。
正常血压大鼠（NTR）和自发性高血压大鼠（SHR）每天接受一次口服 DGP（0.1mg/kg）、氢氯噻嗪（10mg/kg）或载体（10ml/kg）治疗，共 7 天。收集尿液、血液和肾脏样本进行进一步分析。
接受 DGP 治疗的大鼠尿液和 Na+排泄量明显增加。此外，在 DGP 治疗组的尿液中检测到钙节约作用，这与草酸钙晶体形成减少一致。相关地，该治疗并未改变血液中检查的参数。关于肾脏分析，DGP 治疗恢复了 SHR 肾小球区域的形态损伤。除了 NTR 和 SHR 载体组之间观察到的差异外，DGP 增加了还原型谷胱甘肽的含量和谷胱甘肽 S-转移酶 GST 的活性，同时降低了过氧化氢酶和 N-乙酰-β-D-氨基葡萄糖苷酶的活性和亚硝酸盐水平。
综上所述，本研究显示了 DGP 的持久利尿作用及其利钠、保钙和抗尿石作用。DGP 在 SHR 肾脏中的抗氧化和抗炎作用得到了证实，为进一步研究这种黄烷酮的益处提供了前景。
