Bocanegra Rebeca, Ismael Plaza G A, Pulido Carlos R, Ibarra Borja
IMDEA Nanociencia, Faraday 9, Campus Cantoblanco, 28049 Madrid, Spain.
Comput Struct Biotechnol J. 2021 Apr 20;19:2057-2069. doi: 10.1016/j.csbj.2021.04.013. eCollection 2021.
The replisome is the multiprotein molecular machinery that replicates DNA. The replisome components work in precise coordination to unwind the double helix of the DNA and replicate the two strands simultaneously. The study of DNA replication using single-molecule approaches provides a novel quantitative understanding of the dynamics and mechanical principles that govern the operation of the replisome and its components. 'Classical' ensemble-averaging methods cannot obtain this information. Here we describe the main findings obtained with single-molecule methods on the performance of individual replisome components and reconstituted prokaryotic and eukaryotic replisomes. The emerging picture from these studies is that of stochastic, versatile and highly dynamic replisome machinery in which transient protein-protein and protein-DNA associations are responsible for robust DNA replication.
复制体是复制DNA的多蛋白分子机制。复制体组件精确协作，解开DNA双螺旋并同时复制两条链。使用单分子方法研究DNA复制，能对控制复制体及其组件运作的动力学和力学原理提供全新的定量理解。“经典”的总体平均方法无法获得此类信息。在此，我们描述通过单分子方法在单个复制体组件以及重组原核和真核复制体性能方面获得的主要发现。这些研究呈现出的新情况是，复制体机制具有随机性、多功能性和高度动态性，其中瞬时的蛋白质 - 蛋白质和蛋白质 - DNA相互作用负责稳定的DNA复制。
