Mozayeni Mohammad Ali, Vatandoost Farzaneh, Asnaashari Mohammad, Shokri Mehdi, Azari-Marhabi Saranaz, Asnaashari Negin
Department of Endodontics, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Lasers Med Sci. 2020 Fall;11(Suppl 1):S49-S54. doi: 10.34172/jlms.2020.S8. Epub 2020 Dec 30.
Antimicrobial photodynamic therapy (aPDT) as a supplement to the conventional root canal preparation has shown promising results. Previous studies have adopted various combinations of light sources and photosensitizers, which makes it difficult to compare the disinfection efficacy of different PDT protocols. The aim of the present study was to compare the efficacy of three photosensitizers (toluidine blue, methylene blue, and curcumin) in PDT using LED against in root canal disinfection. Root canals of 54 single-rooted extracted teeth were prepared using the ProTaper Gold rotary system and were incubated with for three weeks. They were then randomly divided into five experimental groups and a control group: (1) Irrigation with 2.5% NaOCl for 30 seconds, (2) NaOCl irrigation followed by TB-PDT, (3) NaOCl irrigation followed by MB-PDT, (4) NaOCL irrigation followed by curcumin-PDT, (5) Curcumin solvent (1% ethanol+1% BSA), (6) Control (irrigation with normal saline). Sampling was done by collecting dentin shavings from the root canals, and colony-forming units were determined for each treatment group. The data were analyzed by Kruskal-Wallis and Mann-Whitney U tests. The significance level was set at <0.05. In all treatment groups, the mean values of colony forming unit (CFU) decreased by 99% compared to the control group. The lowest mean values of CFU were observed in groups 2 and 4, followed groups 3, 1, and 5 respectively. The mean CFU count in group 2 was significantly lower than that of group 1 ( value=0.011), while there were no significant differences among groups 1, 3, and 4 ( value >0.05). The adjunction of toluidine blue-mediated PDT by means of a light-emitting diode to NaOCl irrigation increased its antibacterial efficacy against and could be an effective complementary method in root canal disinfection.
抗菌光动力疗法（aPDT）作为传统根管预备的补充方法已显示出有前景的结果。先前的研究采用了各种光源和光敏剂的组合，这使得比较不同光动力疗法方案的消毒效果变得困难。本研究的目的是比较三种光敏剂（甲苯胺蓝、亚甲蓝和姜黄素）在使用发光二极管进行光动力疗法时对根管消毒的效果。使用ProTaper Gold旋转系统对54颗单根离体牙的根管进行预备，并与[此处原文缺失相关物质]一起孵育三周。然后将它们随机分为五个实验组和一个对照组：（1）用2.5%次氯酸钠冲洗30秒，（2）次氯酸钠冲洗后进行甲苯胺蓝光动力疗法（TB-PDT），（3）次氯酸钠冲洗后进行亚甲蓝光动力疗法（MB-PDT），（4）次氯酸钠冲洗后进行姜黄素光动力疗法（curcumin-PDT），（5）姜黄素溶剂（1%乙醇 + 1%牛血清白蛋白），（6）对照组（用生理盐水冲洗）。通过从根管中收集牙本质碎屑进行取样，并测定每个治疗组的菌落形成单位。数据采用Kruskal-Wallis和Mann-Whitney U检验进行分析。显著性水平设定为<0.05。在所有治疗组中，菌落形成单位（CFU）的平均值与对照组相比下降了99%。CFU平均值最低的是第2组和第4组，其次分别是第3组、第1组和第5组。第2组的平均CFU计数显著低于第1组（P值 = 0.011），而第1组、第3组和第4组之间无显著差异（P值>0.05）。通过发光二极管将甲苯胺蓝介导的光动力疗法与次氯酸钠冲洗相结合，可提高其对[此处原文缺失相关细菌]的抗菌效果，并且可能是根管消毒中的一种有效补充方法。
