Arjmand Babak, Vafaee Reza, Razzaghi Mohhamadreza, Rezaei-Tavirani Mostafa, Ahmadzadeh Alireza, Rezaei-Tavirani Sina, Hamdieh Mostafa
Cell Therapy and Regenerative Medicine Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Lasers Med Sci. 2020 Fall;11(Suppl 1):S55-S59. doi: 10.34172/jlms.2020.S9. Epub 2020 Dec 30.
Low-level laser therapy (LLLT) is accompanied by protein expression change in the body. There are many efforts to find a clear relationship between the differentially expressed proteins. This study aims to find the central differentiated expressed proteins of plasma after LLLT. Six proteins are extracted from a proteomics study and the network including these query proteins plus 100 first neighbors was constructed. The central proteins were determined based on degree value, betweenness centrality, closeness centrality (CC), and stress (The centrality parameters). Among 106 nodes of the network, 10 proteins were characterized with the most values of degree, betweenness centrality, CC, and stress. These proteins were determined as central proteins in response to LLLT in plasma. Three query proteins, AHSG, FGG, and SERPINA1, plus 7 first neighbors, namely FGA, ALB, KNG1, FN1, APP, TIMP1, and F5, were identified as central proteins which were dysregulated.
低强度激光疗法（LLLT）会伴随体内蛋白质表达的变化。人们为寻找差异表达蛋白质之间的明确关系付出了诸多努力。本研究旨在找出LLLT后血浆中的核心差异表达蛋白质。从一项蛋白质组学研究中提取了六种蛋白质，并构建了包含这些查询蛋白质以及100个第一邻域的网络。基于度值、介数中心性、紧密中心性（CC）和应力（中心性参数）来确定核心蛋白质。在该网络的106个节点中，有10种蛋白质在度、介数中心性、CC和应力方面具有最多的值。这些蛋白质被确定为血浆中对LLLT产生反应的核心蛋白质。三种查询蛋白质，即α-2-HS-糖蛋白（AHSG）、纤维蛋白原γ链（FGG）和丝氨酸蛋白酶抑制剂A1（SERPINA1），以及7个第一邻域，即纤维蛋白原α链（FGA）、白蛋白（ALB）、激肽原1（KNG1）、纤连蛋白1（FN1）、淀粉样前体蛋白（APP）、金属蛋白酶组织抑制因子1（TIMP1）和凝血因子Ⅴ（F5），被鉴定为失调的核心蛋白质。
