Department of Medical and Clinical Biophysics, Faculty of Medicine, of P. J. Šafárik University in Košice, Košice, Slovakia.
Physiol Res. 2019 Dec 30;68(Suppl 4):S399-S404. doi: 10.33549/physiolres.934377.
The laser radiation absorbed by cells induces production of reactive oxygen species (ROS), followed by the development of oxidative stress. Proteins are major targets for ROS due to their abundance in biological systems. The aim of the present pilot study was to examine the effects of transcutaneous laser blood irradiation (TLBI), i.e., low-level laser therapy (LLLT) at 830 nm on plasma proteome in Wistar rats. Rats were irradiated in the heart area (i.e. coronary arteries) daily (i.e., for 9-day period), by commercially available GaAsAl diode laser (Maestro/CCM, Medicom Prague, Czech Republic, lambda=830 nm, power density 450mW/cm(2), daily dose 60,3 J/ cm(2), irradiation time 134 sec). The comparison of blood plasma proteome from irradiated and non-irradiated rats was performed utilizing 2D electrophoresis followed by MALDI TOF/TOF mass spectrometry. LLLT led to a quantitative change in the acute phase proteins with antioxidant protection i.e., haptoglobin (log(2) fold change (FC)=3.5), hemopexin (log(2) FC=0.5), fibrinogen gamma (log2 FC=1.4), alpha-1-antitrypsin (log(2) FC=-2.2), fetuin A (log2 FC=-0.6) and fetuin B (log2 FC=-2.3). In comparison to conventional biochemical methods, the changes in protein levels in blood plasma induced by LLLT offer a deeper insight into the oxidative stress response.
细胞吸收的激光辐射会诱导活性氧（ROS）的产生，随后发展为氧化应激。由于蛋白质在生物系统中丰富，因此它们是 ROS 的主要靶标。本初步研究旨在检查经皮激光血液辐照（TLBI），即 830nm 低水平激光治疗（LLLT）对 Wistar 大鼠血浆蛋白质组的影响。大鼠每天在心脏区域（即冠状动脉）接受辐照（即 9 天周期），使用市售的 GaAsAl 二极管激光（Maestro/CCM，Medicom 布拉格，捷克共和国，lambda=830nm，功率密度 450mW/cm(2)，日剂量 60.3J/cm(2)，辐照时间 134 秒）。利用 2D 电泳结合 MALDI TOF/TOF 质谱法比较辐照和未辐照大鼠的血浆蛋白质组。LLLT 导致具有抗氧化保护作用的急性期蛋白发生定量变化，即触珠蛋白（log(2) 倍数变化 (FC)=3.5）、血红素结合蛋白（log(2) FC=0.5）、纤维蛋白原γ（log2 FC=1.4）、α-1-抗胰蛋白酶（log(2) FC=-2.2）、胎球蛋白 A（log2 FC=-0.6）和胎球蛋白 B（log2 FC=-2.3）。与传统的生化方法相比，LLLT 诱导的血浆蛋白水平变化更深入地了解了氧化应激反应。
