Wang Wenhao, Huang Zhengwei, Li Yanbei, Wang Wenhua, Shi Jiayu, Fu Fangqin, Huang Ying, Pan Xin, Wu Chuanbin
School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, China.
College of Pharmacy, Jinan University, Guangzhou 511443, China.
Acta Pharm Sin B. 2021 Apr;11(4):1030-1046. doi: 10.1016/j.apsb.2020.10.023. Epub 2020 Oct 29.
When nanoparticles were introduced into the biological media, the protein corona would be formed, which endowed the nanoparticles with new bio-identities. Thus, controlling protein corona formation is critical to therapeutic effect. Controlling the particle size is the most feasible method during design, and the influence of media pH which varies with disease condition is quite important. The impact of particle size and pH on bovine serum albumin (BSA) corona formation of solid lipid nanoparticles (SLNs) was studied here. The BSA corona formation of SLNs with increasing particle size (120-480 nm) in pH 6.0 and 7.4 was investigated. Multiple techniques were employed for visualization study, conformational structure study and mechanism study, etc. "BSA corona-caused aggregation" of SLN2‒3 was revealed in pH 6.0 while the dispersed state of SLNs was maintained in pH 7.4, which significantly affected the secondary structure of BSA and cell uptake of SLNs. The main interaction was driven by van der Waals force plus hydrogen bonding in pH 7.4, while by electrostatic attraction in pH 6.0, and size-dependent adsorption was confirmed. This study provides a systematic insight to the understanding of protein corona formation of SLNs.
当纳米颗粒被引入生物介质中时，会形成蛋白质冠层，这赋予了纳米颗粒新的生物特性。因此，控制蛋白质冠层的形成对治疗效果至关重要。在设计过程中，控制粒径是最可行的方法，而随疾病状况变化的介质pH值的影响也非常重要。本文研究了粒径和pH值对固体脂质纳米粒（SLNs）牛血清白蛋白（BSA）冠层形成的影响。研究了在pH 6.0和7.4条件下，粒径增大（120 - 480 nm）的SLNs的BSA冠层形成情况。采用多种技术进行可视化研究、构象结构研究和机理研究等。在pH 6.0时，发现SLN2 - 3出现“BSA冠层引起的聚集”，而在pH 7.4时，SLNs保持分散状态，这显著影响了BSA的二级结构和SLNs的细胞摄取。在pH 7.4时，主要相互作用由范德华力和氢键驱动，而在pH 6.0时由静电引力驱动，并证实了粒径依赖性吸附。本研究为理解SLNs的蛋白质冠层形成提供了系统的见解。
