Ghosh Rituparna, Sarkhel Sumana, Saha Kanchan, Parua Poulami, Chatterjee Upasana, Mana Koushik
Department of Human Physiology, Vidyasagar University, Paschim Midnapore, 721102, West Bengal, India.
Department of Microbiology, Vidyasagar University, Paschim Medinipur, 721102, West Bengal, India.
Toxicol Rep. 2021 Apr 19;8:888-895. doi: 10.1016/j.toxrep.2021.04.006. eCollection 2021.
The potential of silver nanoparticle(AgNP-AS) mediated bark extract of R.Br was investigated in the study.
METHODS & MATERIALS: AgNP-AS was synthesized with respect to optimal temperature, pH of extract. UV-vis, FT-IR, XRD, TEM, SEM studies were used to characterize silver nanoparticles of Linn(AgNP-AS). The potential of AgNP-AS in neutralization of venom lethality, rise in myotoxicity markers(LDH) and proinflammatory cytokines(IL6, TNFα) were evaluated in animal models.
AgNP-AS was synthesized optimally with AgNO (2 mM); extract concentration, 0.2 gm/l (1% w/v); extract (pH 9) and optimal temperature (40 °C). The colour change and synthesis of AgNP-AS was validated by UV-vis analysis at 432 nm. Transmission electron microscopy of AgNP-AS showed that the particle size for AgNP-AS was 14 nm-20 nm. FT-IR revealed peaks at 3445 cm, 1646 cm, 1346 cm and 1108 cm. From the dynamic light scattering studies the hydrodynamic diameter (115.87 nm) and zeta potential(-29.8 mV) were estimated. The EDAX exhibited a peak for silver validating that the synthesized silver was pure. The biosynthesized (AgNP-AS) could significantly neutralize venom(VRV) induced rise in serum lactate dehydrogenase(LDH) and proinflammatory cytokines(IL6, TNFα) in animal models.
The culmination of nanotechnology with herbal medicine might endow with a really constructive tool in coming up with future drugs with fewer toxicity.
本研究考察了银纳米颗粒（AgNP - AS）介导的红千层树皮提取物的潜力。
在提取物的最佳温度、pH条件下合成AgNP - AS。采用紫外 - 可见光谱、傅里叶变换红外光谱、X射线衍射、透射电子显微镜、扫描电子显微镜研究对红千层银纳米颗粒（AgNP - AS）进行表征。在动物模型中评估AgNP - AS中和毒液致死性、肌毒性标志物（乳酸脱氢酶）升高以及促炎细胞因子（白细胞介素6、肿瘤坏死因子α）的潜力。
用硝酸银（2 mM）、提取物浓度0.2 g/l（1% w/v）、提取物（pH 9）和最佳温度（40°C）可最佳合成AgNP - AS。通过432 nm处的紫外 - 可见分析验证了AgNP - AS的颜色变化和合成。AgNP - AS的透射电子显微镜显示其粒径为14 nm - 20 nm。傅里叶变换红外光谱在3445 cm、1646 cm、1346 cm和1108 cm处显示出峰。通过动态光散射研究估计了流体动力学直径（115.87 nm）和zeta电位（ - 29.8 mV）。能量散射X射线光谱显示银的峰，验证了合成的银是纯的。生物合成的（AgNP - AS）可在动物模型中显著中和毒液（VRV）诱导的血清乳酸脱氢酶（LDH）升高和促炎细胞因子（白细胞介素6、肿瘤坏死因子α）。
纳米技术与草药的结合可能为开发毒性更小的未来药物提供一个非常有建设性的工具。
