Pires Luana C, Guastaldi Fernando P S, Nogueira Andressa V B, Oliveira Nilson T C, Guastaldi Antonio C, Cirelli Joni A
Department of Diagnosis and Surgery, School of Dentistry at Araraquara, Sao Paulo State University-UNESP, Araraquara, SP, Brazil.
Interfacial Electrochemistry Laboratory, Institute of Chemistry of São Carlos, USP, São Carlos, SP, Brazil.
Lasers Med Sci. 2019 Apr;34(3):537-546. doi: 10.1007/s10103-018-2626-2. Epub 2018 Sep 27.
Perform a physicochemical and morphological characterization of a Ti-15Mo alloy surface modified by laser beam irradiation and to evaluate in vitro the morphological response and proliferation of osteoblastic cells seeded onto this alloy. Disks were made of two different metals, Ti-15Mo alloy and cpTi, used as control. A total of four groups were evaluated: polished cpTi (cpTi-pol), laser-irradiated cpTi (cpTi-L), polished Ti-15Mo alloy (Ti-15Mo-pol), and laser-irradiated Ti-15Mo alloy (Ti-15Mo-L). Before and after laser irradiation of the surfaces, physicochemical and morphological analyses were performed: scanning electron microscopy (FEG-SEM), energy-dispersive spectroscopy (EDX), and X-ray diffraction (XRD). The wettability of the samples was evaluated by contact angle measurement. Murine preosteoblastic cells MC3T3-E1 were cultured onto the experimental disks for cell proliferation, morphology, and spreading analyses. Laser groups presented irregular-shaped cavities on its surface and a typical microstructured surface with large depressions (FEG-SEM). The contact angle for both laser groups was 0°, whereas for the polished groups was ≈ 77 and ≈ 78 for cpTi-pol and Ti-15Mo-pol, respectively. Cell proliferation analysis demonstrated a higher metabolic activity in the laser groups (p < 0.05). From the fluorescence microscopy, Ti-15Mo-L surface seems to induce greater cellular differentiation compared to the cpTi-L surface. The preliminary biological in vitro analyses suggested possible advantages of laser surface treatment in the Ti-15Mo alloy regarding cell proliferation and maturation.
对经激光束辐照改性的Ti-15Mo合金表面进行物理化学和形态学表征，并在体外评估接种在该合金上的成骨细胞的形态反应和增殖情况。圆盘由两种不同的金属制成，Ti-15Mo合金和商业纯钛（cpTi）用作对照。总共评估了四组：抛光的cpTi（cpTi-pol）、激光辐照的cpTi（cpTi-L）、抛光的Ti-15Mo合金（Ti-15Mo-pol）和激光辐照的Ti-15Mo合金（Ti-15Mo-L）。在对表面进行激光辐照之前和之后，进行了物理化学和形态学分析：扫描电子显微镜（场发射扫描电子显微镜，FEG-SEM）、能量色散光谱（EDX）和X射线衍射（XRD）。通过接触角测量评估样品的润湿性。将小鼠前成骨细胞MC3T3-E1接种在实验圆盘上，进行细胞增殖、形态和铺展分析。激光处理组的表面呈现出不规则形状的空洞以及带有大凹陷的典型微结构表面（FEG-SEM）。两个激光处理组的接触角均为0°，而抛光组的接触角，cpTi-pol约为77°，Ti-15Mo-pol约为78°。细胞增殖分析表明，激光处理组具有更高的代谢活性（p < 0.05）。从荧光显微镜观察结果来看，与cpTi-L表面相比，Ti-15Mo-L表面似乎能诱导更大程度的细胞分化。初步的体外生物学分析表明，激光表面处理在Ti-15Mo合金的细胞增殖和成熟方面可能具有优势。
