Santos Ana Flávia Piquera, da Silva Rodrigo Capalbo, Hadad Henrique, de Jesus Laís Kawamata, Pereira-Silva Maísa, Nímia Heloisa Helena, Oliveira Sandra Helena Penha, Guastaldi Antônio Carlos, Queiroz Thallita Pereira, Poli Pier Paolo, Barbosa Debora de Barros, da Silva Fabris André Luis, Garcia Júnior Idelmo Rangel, Gruber Reinhard, Souza Francisley Ávila
Department of Diagnosis and Surgery, School of Dentistry, São Paulo State University (UNESP), Araçatuba 16015-050, SP, Brazil.
Department of Dental Materials and Prosthetics, School of Dentistry, São Paulo State University (UNESP), Araçatuba 16015-050, SP, Brazil.
Biology (Basel). 2024 Jul 17;13(7):533. doi: 10.3390/biology13070533.
(1) Objective: The aim of this study was to assess the biological behavior of bone tissue on a machined surface (MS) and modifications made by a laser beam (LS) and by a laser beam incorporated with hydroxyapatite (HA) using a biomimetic method without thermic treatment (LHS). (2) Methods: Scanning electron microscopy coupled with energy-dispersive X-ray spectrometry (SEM/EDX) was performed before and after installation in the rabbit tibiae. A total of 20 Albinus rabbits randomly received 30 implants of 3.75 × 10 mm in the right and left tibias, with two implants on each surface in each tibia. In the animals belonging to the 4-week euthanasia period group, intramuscular application of the fluorochromes calcein and alizarin was performed. In implants placed mesially in the tibiofemoral joint, biomechanical analysis was performed by means of a removal torque (N/cm). The tibias with the implants located distally to the joint were submitted for analysis by confocal laser microscopy (mineral apposition rate) and for histometric analysis by bone contact implant (%BIC) and newly formed bone area (%NBA). (3) Results: The SEM showed differences between the surfaces. The biomechanical analysis revealed significant differences in removal torque values between the MSs and LHSs over a 2-week period. Over a 4-week period, both the LSs and LHSs demonstrated removal torque values statistically higher than the MSs. BIC of the LHS implants were statistically superior to MS at the 2-week period and LHS and LS surfaces were statistically superior to MS at the 4-week period. Statistical analysis of the NBA of the implants showed difference between the LHS and MS in the period of 2 weeks. (4) Conclusions: The modifications of the LSs and LHSs provided important physicochemical modifications that favored the deposition of bone tissue on the surface of the implants.
(1) 目的：本研究旨在评估经机械加工表面（MS）、激光束处理表面（LS）以及采用仿生方法且未经热处理的含羟基磷灰石激光束处理表面（LHS）的骨组织生物学行为及表面改性情况。(2) 方法：在植入兔胫骨前后进行扫描电子显微镜结合能量色散X射线光谱分析（SEM/EDX）。总共20只白化兔在左右胫骨随机植入30枚3.75×10毫米的种植体，每侧胫骨每个表面植入2枚。在属于4周安乐死期组的动物中，进行荧光素钙和茜素的肌肉注射。对于置于胫股关节内侧的种植体，通过去除扭矩（N/cm）进行生物力学分析。对于位于关节远端的带有种植体的胫骨，通过共聚焦激光显微镜进行分析（矿物质沉积率），并通过骨接触种植体（%BIC）和新形成骨面积（%NBA）进行组织计量学分析。(3) 结果：SEM显示各表面之间存在差异。生物力学分析表明，在2周期间，MS和LHS的去除扭矩值存在显著差异。在4周期间，LS和LHS的去除扭矩值在统计学上均高于MS。LHS种植体的%BIC在2周时在统计学上优于MS，LHS和LS表面在4周时在统计学上优于MS。种植体%NBA的统计分析显示，在2周期间LHS和MS之间存在差异。(4) 结论：LS和LHS的改性提供了重要的物理化学改性，有利于骨组织在种植体表面的沉积。
