Kim N P, Ovchinnikov I V, Guliamov D S, Andres Iu P
Vopr Med Khim. 1988 Mar-Apr;34(2):99-102.
Content of glucose, lactate, pyruvate and cAMP was estimated in biopsy from left atrium of 120 patients with the III and IV degree of mitral valve stenosis. Content of 14CO2, developed after incubation of myocardium homogenates with labelled substrates--glucose, glucose-I-phosphate, glucose-6-phosphate, succinate, lactate, was also measured. In patients with the IV degree of mitral valve stenosis concentration of carbohydrates and cAMP was decreased in myocardium as compared with those of patients with the III degree of the disease, whereas consumption of labelled glucose, lactate and succinate was increased. Estimation of the oxidation rate of glucose phosphorylated derivatives enabled to suggest that main part of glucose carbon was involved in reactions of the Krebs cycle. At the same time, a decrease in the content of carbohydrate substrates in myocardium of patients with the IV degree of the stenosis occurred due to elevated rate of these substrates oxidation. The data obtained suggest that adaptation reactions to hemodynamic impairments were responsible for dissimilar character of glucose and lactate metabolism in myocardium of patients with various degree of mitral valve stenosis.
对120例二尖瓣狭窄III度和IV度患者左心房活检组织中的葡萄糖、乳酸、丙酮酸和环磷酸腺苷（cAMP）含量进行了测定。还测量了心肌匀浆与标记底物（葡萄糖、1-磷酸葡萄糖、6-磷酸葡萄糖、琥珀酸、乳酸）孵育后产生的14CO2含量。与二尖瓣狭窄III度患者相比，IV度二尖瓣狭窄患者心肌中的碳水化合物和cAMP浓度降低，而标记葡萄糖、乳酸和琥珀酸的消耗量增加。对葡萄糖磷酸化衍生物氧化速率的估计表明，葡萄糖碳的主要部分参与了三羧酸循环反应。同时，IV度狭窄患者心肌中碳水化合物底物含量的降低是由于这些底物氧化速率升高所致。所获得的数据表明，对血流动力学损害的适应性反应是导致不同程度二尖瓣狭窄患者心肌中葡萄糖和乳酸代谢特征不同的原因。
