Institute of Tissue Regeneration, College of Medicine, Soonchunhyang University, Cheonan, Chung-nam 31151, Republic of Korea.
Department of Microbiology, College of Medicine, Soonchunhyang University, Cheonan, Chung-nam 31151, Republic of Korea.
J Agric Food Chem. 2021 Jun 2;69(21):6032-6042. doi: 10.1021/acs.jafc.1c01440. Epub 2021 May 19.
Although the health benefits of probiotics have been widely known for decades, there has still been limited use of probiotic bacteria in anti-obesity therapy. Herein, we demonstrated the role of subsp. YB0411 (YB, which was selected by an adipogenesis assay) in adipogenic differentiation in 3T3-L1 pre-adipocytes. We observed that YB-treatment effectively reduced triglyceride accumulation and the expression of CCAAT/enhancer-binding protein α, β, and δ (C/EBPα, C/EBPβ, and C/EBPδ), peroxisome proliferator-activated receptor γ (PPARγ), fatty acid-binding protein 4 (aP2), and acetyl-CoA carboxylase (ACC). YB-treatment also reduced the levels of core autophagic markers (p62 and LC3B) in 3T3-L1 pre-adipocytes. Small-interfering-RNA-mediated knockdown and competitive-chemical-inhibition assays showed that AMP-activated protein kinase (AMPK) commenced the anti-adipogenic effect of YB. In addition, YB supplement markedly reduced body weight and fat accretion in mice with high-fat-diet-induced obesity. Our findings suggest that YB may be used as a potential probiotic candidate to ameliorate obesity.
尽管益生菌对健康的益处已经广为人知数十年，但益生菌在抗肥胖治疗中的应用仍然有限。在此，我们研究了 subsp. YB0411 (YB，通过脂肪生成测定法筛选得到) 在 3T3-L1 前脂肪细胞的脂肪生成分化中的作用。我们观察到 YB 处理可有效减少甘油三酯的积累和 CCAAT/增强子结合蛋白 α、β 和 δ (C/EBPα、C/EBPβ 和 C/EBPδ)、过氧化物酶体增殖物激活受体 γ (PPARγ)、脂肪酸结合蛋白 4 (aP2) 和乙酰辅酶 A 羧化酶 (ACC) 的表达。YB 处理还降低了 3T3-L1 前脂肪细胞中核心自噬标志物 (p62 和 LC3B) 的水平。小干扰 RNA 介导的敲低和竞争性化学抑制试验表明，AMP 激活的蛋白激酶 (AMPK) 启动了 YB 的抗脂肪生成作用。此外，YB 补充剂显著降低了高脂肪饮食诱导肥胖小鼠的体重和脂肪堆积。我们的研究结果表明，YB 可能被用作一种潜在的益生菌候选物来改善肥胖。
