Bell J D, Brown J C, Kubal G, Sadler P J
Department of Chemistry, Birkbeck College, University of London, England.
FEBS Lett. 1988 Aug 1;235(1-2):81-6. doi: 10.1016/0014-5793(88)81238-9.
Resonances for lactate are broadened in 500 MHz 1H NMR spectra of human blood plasma and only about one-third is visible in Hahn spin-echo spectra. Similar effects are observed for some other carboxylate anions. Lactate added to the high-Mr fraction of plasma can give rise to peaks which are too broad to observe in either single-pulse or spin-echo spectra. Addition of agents such as NH4Cl of SDS dramatically increases the intensities of lactate peaks. Some glycoproteins appear to broaden lactate resonances.
在人体血浆的500兆赫氢核磁共振谱中，乳酸的共振峰变宽，在哈恩自旋回波谱中只有约三分之一可见。其他一些羧酸根阴离子也观察到类似的效应。添加到血浆高分子量部分的乳酸会产生在单脉冲或自旋回波谱中都太宽而无法观察到的峰。添加诸如氯化铵或十二烷基硫酸钠之类的试剂会显著增加乳酸峰的强度。一些糖蛋白似乎会使乳酸的共振峰变宽。
