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大鼠和人肝细胞培养物中脂肪酸摄取并代谢为酮体和甘油三酯的过程取决于脂肪酸的链长和饱和度。肉碱和胰高血糖素的作用。

Fatty acid uptake and metabolism to ketone bodies and triacyglycerol in rat and human hepatocyte cultures is dependent on chain-length and degree of saturation. Effects of carnitine and glucagon.

作者信息

Emmison N, Agius L

机构信息

Department of Medicine, University of Newcastle upon Tyne, England.

出版信息

FEBS Lett. 1988 Aug 15;236(1):83-8. doi: 10.1016/0014-5793(88)80289-8.

DOI:10.1016/0014-5793(88)80289-8
PMID:3402619
Abstract

Rat and human hepatocyte cultures were incubated with 5 common plasma longchain fatty acids (C16-C18). Rates of fatty acid uptake were similar in rat and human hepatocytes and were of the order: 16:1 greater than 16:0; 18:2 greater than 18:1 greater than 18:0. Rates of ketogenesis were lower in human compared to rat hepatocytes. In rat hepatocytes glucagon stimulated ketogenesis only in the presence of exogenous carnitine and rates of ketogenesis were higher from unsaturated compared to corresponding saturated fatty acids. Glucagon decreased triacylglycerol secretion irrespective of the fatty acid substrate and it increased intracellular triacylglycerol accumulation. The latter effect of glucagon was more marked in the absence of carnitine supplementation.

摘要

将大鼠和人肝细胞培养物与5种常见的血浆长链脂肪酸（C16 - C18）一起孵育。大鼠和人肝细胞中脂肪酸摄取速率相似，顺序为：16:1大于16:0；18:2大于18:1大于18:0。与人肝细胞相比，大鼠肝细胞中酮生成速率较低。在大鼠肝细胞中，胰高血糖素仅在存在外源性肉碱的情况下刺激酮生成，并且与相应的饱和脂肪酸相比，不饱和脂肪酸的酮生成速率更高。无论脂肪酸底物如何，胰高血糖素均降低三酰甘油分泌，并增加细胞内三酰甘油积累。在不补充肉碱的情况下，胰高血糖素的后一种作用更为明显。

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