Guo Changsheng, Hua Yuanqing, Qian Zuanhao
Department of Pediatrics, Affiliated Taikang Xianlin Drum Tower Hospital, Medical School of Nanjing University, Nanjing, China.
Nanjing Maigaoqiao Community Health Service Center, Nanjing, China.
PeerJ. 2021 May 12;9:e11169. doi: 10.7717/peerj.11169. eCollection 2021.
Kawasaki disease (KD) is an acute and febrile systemic vasculitis of unknown etiology. This study aimed to identify the competing endogenous RNA (ceRNA) networks of lncRNAs, miRNAs, and genes in KD and explore the molecular mechanisms underlying KD.
GSE68004 and GSE73464 datasets were downloaded from the Gene Expression Omnibus. Differentially expressed lncRNAs (DElncRNAs) and genes (DEGs) in KD were identified using the criteria of < 0.05 and | log (fold change) | ≥ 1. MicroRNAs (miRNAs) related to KD were searched from databases. The lncRNA-miRNA-mRNA networks involving the DElncRNAs and DEGs were constructed.
A total of 769 common upregulated, 406 common downregulated DEGs, and six DElncRNAs were identified in the KD samples. The lncRNA-miRNA-mRNA network consisted of four miRNAs, three lncRNAs (including the upregulated , , and the downregulated ) and four DEGs (including the downregulated and the upregulated , , and ). Validation in the GSE18606 dataset showed that intravenous immunoglobulin treatment significantly alleviated the deregulated profiles of the above RNAs in KD patients. Three ceRNA networks of , -, and /--- were identified. Four genes were associated with functional categories, such as inflammatory response and vascular endothelial cell.
The ceRNA networks involve genes, such as , , and , and lncRNAs, including , , and , which might play a key role in the pathogenesis and development of KD by regulating inflammation.
川崎病（KD）是一种病因不明的急性发热性全身性血管炎。本研究旨在识别KD中lncRNA、miRNA和基因的竞争性内源性RNA（ceRNA）网络，并探讨KD潜在的分子机制。
从基因表达综合数据库下载GSE68004和GSE73464数据集。使用<0.05和|log（倍数变化）|≥1的标准识别KD中差异表达的lncRNA（DElncRNA）和基因（DEG）。从数据库中搜索与KD相关的微小RNA（miRNA）。构建涉及DElncRNA和DEG的lncRNA-miRNA-mRNA网络。
在KD样本中总共鉴定出769个共同上调、406个共同下调的DEG和6个DElncRNA。lncRNA-miRNA-mRNA网络由4个miRNA、3个lncRNA（包括上调的、和下调的）和4个DEG（包括下调的和上调的、、）组成。在GSE18606数据集中进行验证表明，静脉注射免疫球蛋白治疗显著缓解了KD患者上述RNA的失调情况。鉴定出、-和/---三个ceRNA网络。4个基因与炎症反应和血管内皮细胞等功能类别相关。
ceRNA网络涉及、、等基因以及、、等lncRNA，它们可能通过调节炎症在KD的发病机制和发展中起关键作用。
