Deyneko Dina V, Lebedev Vladimir N, Barbaro Katia, Titkov Vladimir V, Lazoryak Bogdan I, Fadeeva Inna V, Gosteva Alevtina N, Udyanskaya Irina L, Aksenov Sergey M, Rau Julietta V
Chemistry Department, Lomonosov Moscow State University, Leninskie Gory 1, 119991 Moscow, Russia.
Laboratory of Arctic Mineralogy and Material Sciences, Kola Science Centre RAS, 14 Fersman Str., 184209 Apatity, Russia.
Biomimetics (Basel). 2023 Dec 31;9(1):14. doi: 10.3390/biomimetics9010014.
β-Tricalcium phosphate (β-TCP) is widely used as bone implant material. It has been observed that doping the β-TCP structure with certain cations can help in combating bacteria and pathogenic microorganisms. Previous literature investigations have focused on tricalcium phosphate structures with silver, copper, zinc, and iron cations. However, there are limited studies available on the biological properties of β-TCP containing nickel and cobalt ions. In this work, CaNi(PO) and CaCo(PO) solid solutions with the β-Ca(PO) structure were synthesized by a high-temperature solid-state reaction. Structural studies revealed the β-TCP structure becomes saturated at 9.5 mol/% for Co or Ni ions. Beyond this saturation point, Ni and Co ions form impurity phases after complete occupying of the octahedral M5 site. The incorporation of these ions into the β-TCP crystal structure delays the phase transition to the α-TCP phase and stabilizes the structure as the temperature increases. Biocompatibility tests conducted on adipose tissue-derived mesenchymal stem cells (aMSC) using the (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) (MTT) assay showed that all prepared samples did not exhibit cytotoxic effects. Furthermore, there was no inhibition of cell differentiation into the osteogenic lineage. Antibacterial properties were studied on the fungus and on , , and bacteria strains. The Ni- and Co-doped β-TCP series exhibited varying degrees of bacterial growth inhibition depending on the doping ion concentration and the specific bacteria strain or fungus. The combination of antibacterial activity and cell-friendly properties makes these phosphates promising candidates for anti-infection bone substitute materials.
β-磷酸三钙（β-TCP）被广泛用作骨植入材料。据观察，用某些阳离子掺杂β-TCP结构有助于对抗细菌和致病微生物。先前的文献研究主要集中在含有银、铜、锌和铁阳离子的磷酸三钙结构上。然而，关于含镍和钴离子的β-TCP生物学特性的研究有限。在这项工作中，通过高温固态反应合成了具有β-Ca(PO)结构的CaNi(PO)和CaCo(PO)固溶体。结构研究表明，对于钴或镍离子，β-TCP结构在9.5摩尔/%时达到饱和。超过这个饱和点，镍和钴离子在完全占据八面体M5位点后形成杂质相。随着温度升高，这些离子掺入β-TCP晶体结构会延迟向α-TCP相的相变并使结构稳定。使用（3-[4,5-二甲基噻唑-2-基]-2,5-二苯基溴化四氮唑）（MTT）法对脂肪组织来源的间充质干细胞（aMSC）进行的生物相容性测试表明，所有制备的样品均未表现出细胞毒性作用。此外，也没有抑制细胞向成骨谱系分化。对真菌以及金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌和白色念珠菌菌株进行了抗菌性能研究。镍和钴掺杂的β-TCP系列根据掺杂离子浓度和特定细菌菌株或真菌表现出不同程度的细菌生长抑制。抗菌活性和细胞友好特性的结合使这些磷酸盐成为抗感染骨替代材料的有前景的候选者。
