Golubchikov Daniil O, Fadeeva Inna V, Knot'ko Alexander V, Kostykov Iliya A, Slonskaya Tatiana K, Barbaro Katia, Zepparoni Alessia, Fosca Marco, Antoniac Iulian V, Rau Julietta V
Chemistry Department, Lomonosov Moscow State University, Leninskie Gory 1, 119991 Moscow, Russia.
Department of Materials Science, Lomonosov Moscow State University, Leninskie Gory 1, 119991 Moscow, Russia.
Molecules. 2025 Mar 31;30(7):1575. doi: 10.3390/molecules30071575.
Current research in bone tissue engineering is focused not only on basic parameters of the materials, such as biocompatibility and degradation rate but also on intrinsic osteogenic and antimicrobial properties, essential to provide a rapid tissue regeneration without negative effects due to periprosthetic infections, that may result in revision surgeries. One of the major strategies to enhance the osteogenic and antimicrobial performance of calcium phosphates is the ionic substitution, in particular, with magnesium and borates. In this study, we focused on the synthesis of boron-substituted tricalcium phosphate (B-TCP) with a target of 5 mol.% substitution via the solid-state synthesis with mechano-activation. Synthesis from raw precursors, without the preliminary brushite wet precipitation, led to the primary phase of β-TCP, which was proved by the XRD analysis. According to the IR-spectroscopy and P NMR analysis, boron substitution occurred in the synthesized sample. The developed material showed a modest antibacterial performance against , with 13.5 ± 5.0% growth inhibition, and , with 16.7 ± 5.5% inhibition. The biocompatibility of β-TCP and B-TCP was tested through the MTT assay and osteogenic differentiation of the mesenchymal stromal cells. The proposed synthesis approach can be useful for the fabrication of B-TCP ceramics for bone tissue engineering.
目前骨组织工程的研究不仅集中在材料的基本参数上，如生物相容性和降解速率，还集中在内在的成骨和抗菌性能上，这些性能对于在不产生因假体周围感染导致的负面影响（可能需要进行翻修手术）的情况下实现快速组织再生至关重要。提高磷酸钙成骨和抗菌性能的主要策略之一是离子取代，特别是用镁和硼酸盐进行取代。在本研究中，我们专注于通过机械活化的固态合成法合成硼取代度为5摩尔%的硼取代磷酸三钙（B-TCP）。从原始前驱体直接合成，无需预先进行透钙磷石湿法沉淀，得到了β-TCP主相，这通过XRD分析得到证实。根据红外光谱和磷核磁共振分析，硼取代发生在合成样品中。所开发的材料对[具体细菌1]表现出适度的抗菌性能，生长抑制率为13.5±5.0%，对[具体细菌2]的生长抑制率为16.7±5.5%。通过MTT法和间充质基质细胞的成骨分化测试了β-TCP和B-TCP的生物相容性。所提出的合成方法可用于制备用于骨组织工程的B-TCP陶瓷。
