Centre for Integrative Biological Signalling Studies CISA, University of Freiburg, Habsburger Straße 49, 79104, Freiburg, Germany.
Hermann Staudinger Graduate School, University of Freiburg, Hebelstr. 27, 79104, Freiburg, Germany.
Metabolomics. 2021 May 24;17(6):52. doi: 10.1007/s11306-021-01799-y.
One approach to dampen the inflammatory reactions resulting from implantation surgery of cochlear implant hearing aids is to embed dexamethasone into the matrix of the electrode carrier. Possible side effects for sensory cells in the inner ear on the metabolomics have not yet been evaluated.
We examined changes in the metabolome of the HEI-OC1 cell line after dexamethasone incubation as a cell model of sensory cells of the inner ear.
Untargeted GC-MS-profiling of metabolic alterations after dexamethasone treatment showed that dexamethasone had antithetical effects on the metabolic signature of the cells depending on growth conditions. The differentiated state of HEI-OC1 cells is better suited for elucidating metabolic changes induced by external factors. Dexamethasone treatment of differentiated cells led to an increase in intracellular amino acids and enhanced glucose uptake and β-oxidation in the cells. Increased availability of precursors for glycolysis and ATP production by β-oxidation stabilizes the energy supply in the cells, which could be assumed to be beneficial in coping with cellular stress. We found no negative effects of dexamethasone on the metabolic level, and changes may even prepare sensory cells to better overcome cellular stress following implantation surgery.
为了减轻人工耳蜗植入手术引起的炎症反应，一种方法是将地塞米松嵌入电极载体的基质中。尚未评估内耳感觉细胞的代谢组学上的这种地塞米松可能产生的副作用。
我们以内耳感觉细胞的 HEI-OC1 细胞系作为细胞模型，研究了地塞米松孵育后细胞代谢组的变化。
对地塞米松处理后代谢变化的非靶向 GC-MS 分析表明，地塞米松对细胞代谢特征的影响取决于生长条件。分化状态的 HEI-OC1 细胞更适合阐明外源性因素诱导的代谢变化。地塞米松处理分化细胞导致细胞内氨基酸增加，葡萄糖摄取和细胞内 β-氧化增强。β-氧化增加糖酵解和 ATP 产生的前体，稳定细胞的能量供应，这可以假设为应对细胞应激提供了益处。我们在地塞米松的代谢水平上没有发现负面效应，而且这种变化甚至可能使感觉细胞在植入手术后更好地克服细胞应激。
