Tian Yi, Gao Zhiyong, Li Weihong, Liu Baiwei, Chen Yanwei, Jia Lei, Yan Hanqiu, Wang Quanyi
Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control and Beijing Research Center for Preventive Medicine, Beijing, China.
J Med Virol. 2021 Nov;93(11):6191-6199. doi: 10.1002/jmv.27100. Epub 2021 Jun 8.
Group A rotavirus (RVA) is one of the most common causes of severe diarrhea in children worldwide. However, RVA is also an important pathogen causing adult diarrhea, with higher infection rates in older patients. To provide evidence for rotavirus epidemic control and to inform vaccine development, we analyzed the molecular epidemiology of RVA among adult outpatients with diarrhea in Beijing from 2011 to 2018. Stool specimens were collected monthly from 14 districts. RVA was detected using enzyme-linked immunosorbent assay and real-time reverse-transcription polymerase chain reaction (RT-PCR). Genotyping of rotavirus was performed using multiplex semi-nested RT-PCR. Phylogenetic analysis was performed using maximum likelihood methods implemented in MEGA software (version 6.06). Logistic regression and chi-square tests were used to assess differences among age groups, districts, years, and genotype distributions. The prevalence of rotavirus was 10.16% (1310 of 12,893) among adult outpatients with diarrhea from 2011 to 2018 in Beijing. The highest prevalence (13.74%, 600 of 4367) was observed among those aged 41 to 65 years. November, December, and January had the highest positive detection rates. In 2011, G3P[8] and G9P[8] were the dominant genotypes. Starting from 2012, G9P[8] became the dominant genotype. Most G9 strains belonged to the G9-VI clade. Most P[8] strains belonged to the P[8]-III clade. RVA is a major cause of adult diarrhea in Beijing. Continuous molecular surveillance is needed, and transmission of rotavirus between children and adults should be investigated further.
A组轮状病毒（RVA）是全球儿童严重腹泻最常见的病因之一。然而，RVA也是导致成人腹泻的重要病原体，在老年患者中的感染率更高。为了提供轮状病毒疫情防控的证据并为疫苗研发提供信息，我们分析了2011年至2018年北京成人腹泻门诊患者中RVA的分子流行病学。每月从14个区收集粪便标本。使用酶联免疫吸附测定和实时逆转录聚合酶链反应（RT-PCR）检测RVA。使用多重半巢式RT-PCR进行轮状病毒基因分型。使用MEGA软件（6.06版）中实施的最大似然法进行系统发育分析。使用逻辑回归和卡方检验评估年龄组、区、年份和基因型分布之间的差异。2011年至2018年北京成人腹泻门诊患者中轮状病毒的患病率为10.16%（12893例中的1310例）。41至65岁人群的患病率最高（13.74%，4367例中的600例）。11月、12月和1月的阳性检出率最高。2011年，G3P[8]和G9P[8]是主要基因型。从2012年开始，G9P[8]成为主要基因型。大多数G9毒株属于G9-VI分支。大多数P[8]毒株属于P[8]-III分支。RVA是北京成人腹泻的主要病因。需要持续进行分子监测，并应进一步调查轮状病毒在儿童和成人之间的传播情况。
