Moroux Y, Boschetti E, Bouvet J P, Barot-Ciorbaru R
IBF Biotechnics, Villeneuve-la-Garenne, France.
J Chromatogr. 1988 May 25;440:119-30. doi: 10.1016/s0021-9673(00)94516-3.
Cell walls from Nocardia opaca induce the production of mitogenic factors by mouse peritoneal macrophages in vitro. These factors stimulate thymocytes from C3H/HeJ mice. Supernatants of peritoneal cell culture exhibiting this activity were fractionated by chromatographic procedures such as gel filtration and metal chelate affinity chromatography and the biological activities assayed. These fractionation studies indicate that several biologically active products occur in the supernatant. Four factors monokines (M) with different apparent molecular masses M1 (100,000), M2 (50,000), M3 (16,000) and M4 (7000) were obtained, one of which (M3) was identical to interleukin 1 (IL1). Several of the biochemical parameters of one of these factors, M2, were analyzed. It was found that this monokine had many properties in common with IL1: stimulation of proliferation of thymocytes from C3H/HeJ mice, similar amino acid composition and mobility during isoelectric focusing.
不透明诺卡菌的细胞壁可在体外诱导小鼠腹腔巨噬细胞产生促有丝分裂因子。这些因子可刺激C3H/HeJ小鼠的胸腺细胞。对表现出这种活性的腹腔细胞培养上清液进行凝胶过滤和金属螯合亲和色谱等色谱程序分级分离，并测定其生物活性。这些分级分离研究表明，上清液中存在几种生物活性产物。获得了四种具有不同表观分子量的单核因子（M），即M1（100,000）、M2（50,000）、M3（16,000）和M4（7000），其中一种（M3）与白细胞介素1（IL1）相同。对其中一种因子M2的几个生化参数进行了分析。发现这种单核因子与IL1有许多共同特性：刺激C3H/HeJ小鼠胸腺细胞增殖、相似的氨基酸组成以及在等电聚焦过程中的迁移率。
