从多种诺卡氏菌属菌种中分离促有丝分裂和佐剂活性组分。
Isolation of mitogenic and adjuvant active fractions from various species of Nocardiae.
作者信息
Ciorbaru R, Adam A, Petit J F, Lederer E, Bona C, Chedid L
出版信息
Infect Immun. 1975 Feb;11(2):257-64. doi: 10.1128/iai.11.2.257-264.1975.
Abstract
Delipidated lysozyme digests of Nocardia opaca, N. corallina, and N. rubra have been fractionated by Sephadex filtration. The mitogenic and adjuvant activities of the fractions thus obtained have been investigated. All fractions are mitogenic except the last fraction of N.rubra, but the N. opaca products induce a stronger stimulation of mouse spleen lymphocytes than the corresponding fractions of the two other species. The activity of the first Sephadex fractions of each strain has been compared to other mitogens (concanavalin A, lipopolysaccharide). All fractions are adjuvant, although one of them, the last Sephadex fraction of N. rubra, does not contain peptidoglycan; its activity must thus be attributed to another kind of molecule. Fractionation of the first Sephadex fraction of N. opaca by centrifugation in glacial acetic acid led to a separation of adjuvant and mitogenic activities.
摘要
对不透明诺卡氏菌、珊瑚诺卡氏菌和红色诺卡氏菌的脱脂溶菌酶消化物进行了葡聚糖凝胶过滤分级分离。对由此获得的各组分的促有丝分裂活性和佐剂活性进行了研究。除红色诺卡氏菌的最后一个组分外，所有组分均具有促有丝分裂活性，但不透明诺卡氏菌的产物比其他两个菌种的相应组分对小鼠脾淋巴细胞的刺激更强。已将每个菌株的第一个葡聚糖凝胶组分的活性与其他促有丝分裂原（伴刀豆球蛋白A、脂多糖）进行了比较。所有组分均为佐剂，尽管其中之一，即红色诺卡氏菌的最后一个葡聚糖凝胶组分不含肽聚糖；因此其活性必定归因于另一种分子。通过在冰醋酸中离心对不透明诺卡氏菌的第一个葡聚糖凝胶组分进行分级分离，导致佐剂活性和促有丝分裂活性分离。
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