Deubel V, Kinney R M, Esposito J J, Cropp C B, Vorndam A V, Monath T P, Trent D W
Division of Vector-Borne Viral Diseases, Centers for Disease Control, Fort Collins, Colorado 80522.
J Gen Virol. 1988 Aug;69 ( Pt 8):1921-9. doi: 10.1099/0022-1317-69-8-1921.
A cDNA copy of the dengue (DEN) 2 virus genome region encoding the virion capsid, membrane and envelope structural proteins has been inserted into vaccinia virus (VV) DNA under the control of its 11K late promoter. The DEN-2 envelope protein was expressed and processed in cells infected with the VV recombinant (VV/D2S). No DEN-2 virus antibody response was detected in mice, hamsters or monkeys vaccinated with VV/D2S. Furthermore, a viraemia was observed in recombinant-vaccinated monkeys after challenge with infectious DEN-2 virus.
登革热（DEN）2型病毒基因组中编码病毒体衣壳、膜和包膜结构蛋白的区域的互补DNA（cDNA）拷贝，已在痘苗病毒（VV）的11K晚期启动子控制下插入到痘苗病毒（VV）DNA中。DEN - 2包膜蛋白在感染了VV重组体（VV/D2S）的细胞中表达并加工。在用VV/D2S接种的小鼠、仓鼠或猴子中未检测到DEN - 2病毒抗体反应。此外，在用传染性DEN - 2病毒攻击后，在接种重组疫苗的猴子中观察到病毒血症。
