confinement size 决定了 Ran 诱导的微管网络的结构。
Confinement size determines the architecture of Ran-induced microtubule networks.
机构信息
Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ, USA.
Department of Molecular Biology, Princeton University, Princeton, NJ, USA.
出版信息
Soft Matter. 2021 Jun 28;17(24):5921-5931. doi: 10.1039/d1sm00045d. Epub 2021 May 27.
The organization of microtubules (MTs) is critical for cells during interphase and mitosis. During mitotic spindle assembly, MTs are made and organized around chromosomes in a process regulated by RanGTP. The role of RanGTP has been explored in Xenopus egg extracts, which are not limited by a cell membrane. Here, we investigated whether cell-sized confinements affect the assembly of RanGTP-induced MT networks in Xenopus egg extracts. We used microfluidics to encapsulate extracts within monodisperse extract-in-oil droplets. Importantly, we find that the architecture of Ran-induced MT networks depends on the droplet diameter and the Ran concentration, and differs from structures formed in bulk extracts. Our results highlight that both MT nucleation and physical confinement play critical roles in determining the spatial organization of the MT cytoskeleton.
微管（MTs）的组织对于有丝分裂间期和有丝分裂期的细胞至关重要。在有丝分裂纺锤体组装过程中，MTs 在 RanGTP 调节下在染色体周围形成和组织。RanGTP 的作用在爪蟾卵提取物中进行了探索，该提取物不受细胞膜限制。在这里，我们研究了细胞大小的限制是否会影响 RanGTP 诱导的 MT 网络在爪蟾卵提取物中的组装。我们使用微流控技术将提取物封装在单分散的油包水液滴中。重要的是，我们发现 Ran 诱导的 MT 网络的结构取决于液滴直径和 Ran 浓度，并且与在块状提取物中形成的结构不同。我们的结果强调了 MT 成核和物理限制在决定 MT 细胞骨架的空间组织中都起着关键作用。
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