Department of Clinical Laboratory Sciences, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.
Department of Medical Laboratory Science, Tabuk University, Tabuk, Saudi Arabia.
Microb Pathog. 2021 Aug;157:104989. doi: 10.1016/j.micpath.2021.104989. Epub 2021 May 25.
This study assessed the correlation between biofilm formation in Pseudomonas aeruginosa strains with both the level of antibiotic resistance, and the number of virulence- and biofilm-related genes encoded. A total of sixty-six, non-replicate and prospectively collected P. aeruginosa strains were identified and tested. Potential ampD mutations that may impose resistance to extended-spectrum β-lactam (ESBL) agents were further explored. Of the sixty-six tested isolates, 40 demonstrated the multidrug resistance (MDR) phenotype, while twenty-six were non-MDR strains. An inverse correlation was observed between antibiotic resistance and the potential capacity to form biofilms. In addition, no correlation was observed between novel ampD mutations and the tendency for MDR isolates to acquire a β-lactam-resistant phenotype. The present study emphasizes the need for enhanced infection preventive measures in various hospital units, since both MDR and non-MDR P. aeruginosa isolates exhibited a high level of biofilm-forming capacity and the presence of virulence-associated genes.
本研究评估了铜绿假单胞菌菌株生物膜形成与抗生素耐药水平和编码的毒力和生物膜相关基因数量之间的相关性。共鉴定并测试了 66 株非重复前瞻性采集的铜绿假单胞菌菌株。进一步探索了可能导致对扩展谱β-内酰胺（ESBL）药物产生耐药性的潜在 ampD 突变。在 66 株受试分离株中，有 40 株表现出多药耐药（MDR）表型，而 26 株为非 MDR 菌株。抗生素耐药性与生物膜形成的潜在能力呈负相关。此外，新型 ampD 突变与 MDR 分离株获得β-内酰胺耐药表型的趋势之间未观察到相关性。本研究强调了在各个医院单位加强感染预防措施的必要性，因为 MDR 和非 MDR 铜绿假单胞菌分离株均表现出高水平的生物膜形成能力和毒力相关基因的存在。
