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LINC01158通过海绵吸附miR-6734-3p以增强CENPK表达，从而在胶质瘤中发挥癌基因作用。

LINC01158 works as an oncogene in glioma via sponging miR-6734-3p to boost CENPK expression.

作者信息

Sun Zhenxing, Wei Naili, Yao Shenglian, Wang Guihuai, Sun Yaxing, Wang Zhenze, Yuan Dan

机构信息

Department of Neurosurgery, School of Clinical Medicine, Beijing Tsinghua Changgung Hospital, Tsinghua University, Beijing, 102218, China.

Department of Neurosurgery, The First Affiliated Hospital of Shantou University Medical College, Shantou, 515041, Guangdong, China.

出版信息

Cancer Cell Int. 2021 May 27;21(1):280. doi: 10.1186/s12935-021-01931-x.

DOI:10.1186/s12935-021-01931-x
PMID:34044826
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8161569/
Abstract

BACKGROUND

Long non-coding RNAs (lncRNAs) have been certified to play vital biological functions in glioma and have received considerable attention in the recent literature. Nonetheless, the role of LINC01158 in glioma remains to be elucidated.

METHODS

qRT-PCR, western blot and GEPIA database were applied for reporting the expression of CENPK and LINC01158 in glioma and the correlation between LINC01158 and CENPK expression. EdU, colony formation, CCK-8, caspase-3 activity and TUNEL assays probed the impacts of LINC01158 on glioma cell growth. Subcellular fractionation and FISH assays revealed the cellular distribution of LINC01158. Luciferase reporter and RIP assays examined ceRNA network of LINC01158, CENPK and miR-6734-3p.

RESULTS

LINC01158 and CENPK were both overexpressed in glioma and a positive regulation of LINC01158 on CENPK was corroborated. LINC01158 served a pro-proliferative and anti-apoptotic part in glioma by sponging miR-6734-3p to augment CENPK.

CONCLUSION

LINC01158 enhances CENPK by serving as sponge for miR-6734-3p to facilitate glioma development, proposing LINC01158 as a new player in glioma.

摘要

背景

长链非编码RNA（lncRNAs）已被证实可在胶质瘤中发挥重要生物学功能，并在近期文献中受到广泛关注。尽管如此，LINC01158在胶质瘤中的作用仍有待阐明。

方法

采用qRT-PCR、蛋白质免疫印迹法和GEPIA数据库来报告CENPK和LINC01158在胶质瘤中的表达情况以及LINC01158与CENPK表达之间的相关性。EdU、集落形成、CCK-8、caspase-3活性和TUNEL检测探究LINC01158对胶质瘤细胞生长的影响。亚细胞分级分离和FISH检测揭示LINC01158的细胞分布。荧光素酶报告基因和RIP检测研究LINC01158、CENPK和miR-6734-3p的ceRNA网络。

结果

LINC01158和CENPK在胶质瘤中均过表达，且证实LINC01158对CENPK具有正向调控作用。LINC01158通过海绵吸附miR-6734-3p增强CENPK，在胶质瘤中发挥促增殖和抗凋亡作用。

结论

LINC01158作为miR-6734-3p的海绵吸附分子增强CENPK，促进胶质瘤发展，提示LINC01158是胶质瘤中的一个新因子。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/759d/8161569/d5747ee2867e/12935_2021_1931_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/759d/8161569/7045983526a0/12935_2021_1931_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/759d/8161569/6d9f6c9d748f/12935_2021_1931_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/759d/8161569/d0e0276b9780/12935_2021_1931_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/759d/8161569/d5747ee2867e/12935_2021_1931_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/759d/8161569/7045983526a0/12935_2021_1931_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/759d/8161569/6d9f6c9d748f/12935_2021_1931_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/759d/8161569/d0e0276b9780/12935_2021_1931_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/759d/8161569/d5747ee2867e/12935_2021_1931_Fig4_HTML.jpg

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