School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran.
Burn Research Center, Iran University of Medical Sciences, Tehran, Iran.
Carbohydr Polym. 2021 Aug 15;266:118123. doi: 10.1016/j.carbpol.2021.118123. Epub 2021 Apr 28.
In this study, we fabricated two different arrangements of laminated composite scaffolds based on Alginate:Alginate sulfate hydrogel, PCL:Gelatin electrospun mat, and Kartogenin-PLGA nanoparticles (KGN-NPs). The optimized composite scaffold revealed a range of advantages such as improved mechanical features as well as less potential of damage (less dissipated energy), interconnected pores of hydrogel and fiber with adequate pore size, excellent swelling ratio, and controlled biodegradability. Furthermore, the synthesized KGN-NPs with spherical morphology were incorporated into the composite scaffold and exhibited a linear and sustained release of KGN within 30 days with desirable initial burst reduction (12% vs. 20%). Additionally, the cytotoxicity impact of the composite was evaluated. Resazurin assay and Live/Dead staining revealed that the optimized composite scaffold has no cytotoxic effect and could improve cell growth. Overall, according to the enhanced mechanical features, suitable environment for cellular growth, and sustained drug release, the optimized scaffold would be a good candidate for tissue regeneration.
在这项研究中，我们制备了两种不同的层状复合支架，基于藻酸盐：藻酸盐硫酸酯水凝胶、PCL：明胶电纺毡和 Kartogenin-PLGA 纳米颗粒（KGN-NPs）。优化后的复合支架具有一系列优点，例如改善了机械性能，潜在的损伤较小（能量损耗较小），水凝胶和纤维的互连孔具有适当的孔径，优异的溶胀比和可控的生物降解性。此外，合成的具有球形形态的 KGN-NPs 被掺入到复合支架中，并表现出 KGN 的线性和持续释放，在 30 天内达到理想的初始突释减少（12%对 20%）。此外，还评估了复合支架的细胞毒性影响。Resazurin 测定和 Live/Dead 染色显示，优化后的复合支架没有细胞毒性作用，并可以促进细胞生长。总的来说，根据增强的机械性能、适合细胞生长的环境和持续的药物释放，优化后的支架将是组织再生的良好候选物。
