从尼日利亚索科托市山羊中分离出的口疮病毒的分子特征及系统发育分析
Molecular characterization and phylogenetic analysis of orf virus isolated from goats in Sokoto metropolis, Nigeria.
作者信息
Lawal Nafi'u, Ibrahim Mubarak, Onawala Dauda Ayomide, Bello Muhammad Bashir, Aliyu Rabiu Muhammad, Baraya Yusha'u Shu'aibu, Aliyu Abdullahi, Ibrahim Aliyu Musawa, Sa'adu Aliyu
机构信息
Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria.
Central Veterinary Research Laboratory, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria.
出版信息
Future Sci OA. 2021 Apr 20;7(6):FSO700. doi: 10.2144/fsoa-2020-0162.
AIM
The aim of this study was to molecularly characterize orf virus isolated from clinical infections in goats in Sokoto metropolis.
MATERIALS & METHODS: Embryonated chicken eggs were used to isolate orf virus according to the established protocol. Viral DNA was extracted and full coding region of gene was amplified by polymerase chain reaction, sequenced and blasted for identification and phylogenetically analyzed.
RESULTS AND DISCUSSION
The gene sequences of the isolate showed slight variability (96-98.7%) with the reference sequences as it clustered within the same clade with Korean, Zambian and Ethiopian strains, signifying a close genetic relationship. Unique amino acid substitutions were noted. This is the first genetic characterization of gene of orf virus circulating in Nigeria.
CONCLUSION
This study has provided in sight into the genetic diversity of orf virus in the study area.
目的
本研究旨在对从索科托市山羊临床感染中分离出的口疮病毒进行分子特征分析。
材料与方法
按照既定方案，使用鸡胚来分离口疮病毒。提取病毒DNA，通过聚合酶链反应扩增基因的完整编码区，进行测序和比对以进行鉴定，并进行系统发育分析。
结果与讨论
分离株的基因序列与参考序列显示出轻微的变异性（96 - 98.7%），因为它与韩国、赞比亚和埃塞俄比亚菌株聚集在同一进化枝内，表明存在密切的遗传关系。注意到了独特的氨基酸取代。这是尼日利亚流行的口疮病毒基因的首次遗传特征分析。
结论
本研究深入了解了研究区域内口疮病毒的遗传多样性。
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