• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

从尼日利亚索科托市山羊中分离出的口疮病毒的分子特征及系统发育分析

Molecular characterization and phylogenetic analysis of orf virus isolated from goats in Sokoto metropolis, Nigeria.

作者信息

Lawal Nafi'u, Ibrahim Mubarak, Onawala Dauda Ayomide, Bello Muhammad Bashir, Aliyu Rabiu Muhammad, Baraya Yusha'u Shu'aibu, Aliyu Abdullahi, Ibrahim Aliyu Musawa, Sa'adu Aliyu

机构信息

Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria.

Central Veterinary Research Laboratory, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria.

出版信息

Future Sci OA. 2021 Apr 20;7(6):FSO700. doi: 10.2144/fsoa-2020-0162.

DOI:10.2144/fsoa-2020-0162
PMID:34046202
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8147748/
Abstract

AIM

The aim of this study was to molecularly characterize orf virus isolated from clinical infections in goats in Sokoto metropolis.

MATERIALS & METHODS: Embryonated chicken eggs were used to isolate orf virus according to the established protocol. Viral DNA was extracted and full coding region of gene was amplified by polymerase chain reaction, sequenced and blasted for identification and phylogenetically analyzed.

RESULTS AND DISCUSSION

The gene sequences of the isolate showed slight variability (96-98.7%) with the reference sequences as it clustered within the same clade with Korean, Zambian and Ethiopian strains, signifying a close genetic relationship. Unique amino acid substitutions were noted. This is the first genetic characterization of gene of orf virus circulating in Nigeria.

CONCLUSION

This study has provided in sight into the genetic diversity of orf virus in the study area.

摘要

目的

本研究旨在对从索科托市山羊临床感染中分离出的口疮病毒进行分子特征分析。

材料与方法

按照既定方案，使用鸡胚来分离口疮病毒。提取病毒DNA，通过聚合酶链反应扩增基因的完整编码区，进行测序和比对以进行鉴定，并进行系统发育分析。

结果与讨论

分离株的基因序列与参考序列显示出轻微的变异性（96 - 98.7%），因为它与韩国、赞比亚和埃塞俄比亚菌株聚集在同一进化枝内，表明存在密切的遗传关系。注意到了独特的氨基酸取代。这是尼日利亚流行的口疮病毒基因的首次遗传特征分析。

结论

本研究深入了解了研究区域内口疮病毒的遗传多样性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/777f/8147748/192e0721e408/fsoa-07-700-g5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/777f/8147748/9b2c53d1766e/fsoa-07-700-g1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/777f/8147748/8a7be7e613d5/fsoa-07-700-g2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/777f/8147748/d8d510ab57b9/fsoa-07-700-g3a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/777f/8147748/5bca4501032c/fsoa-07-700-g4a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/777f/8147748/192e0721e408/fsoa-07-700-g5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/777f/8147748/9b2c53d1766e/fsoa-07-700-g1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/777f/8147748/8a7be7e613d5/fsoa-07-700-g2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/777f/8147748/d8d510ab57b9/fsoa-07-700-g3a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/777f/8147748/5bca4501032c/fsoa-07-700-g4a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/777f/8147748/192e0721e408/fsoa-07-700-g5.jpg

相似文献

1
Molecular characterization and phylogenetic analysis of orf virus isolated from goats in Sokoto metropolis, Nigeria.从尼日利亚索科托市山羊中分离出的口疮病毒的分子特征及系统发育分析
Future Sci OA. 2021 Apr 20;7(6):FSO700. doi: 10.2144/fsoa-2020-0162.
2
Molecular detection and characterization of Orf virus from goats in Egypt.从埃及山羊中检测和鉴定口疮病毒。
Open Vet J. 2022 Mar-Apr;12(2):273-280. doi: 10.5455/OVJ.2022.v12.i2.16.
3
Identification and phylogenetic analysis of orf virus from goats in Taiwan.台湾山羊中orf病毒的鉴定及系统发育分析
Virus Genes. 2007 Dec;35(3):705-12. doi: 10.1007/s11262-007-0144-6. Epub 2007 Aug 8.
4
Contagious ecthyma in Egypt: Clinical, virological and molecular explorations.埃及的传染性脓疱病：临床、病毒学及分子学研究
Virology. 2024 Jan;589:109924. doi: 10.1016/j.virol.2023.109924. Epub 2023 Nov 7.
5
Phylogenetic analysis and characterization of Korean orf virus from dairy goats: case report.从奶山羊中分离韩国疱疹病毒的进化分析及特征：病例报告。
Virol J. 2009 Oct 16;6:167. doi: 10.1186/1743-422X-6-167.
6
Phylogenetic analysis of two Chinese orf virus isolates based on sequences of B2L and VIR genes.基于 B2L 和 VIR 基因序列对两株中国 ORF 病毒分离株的系统进化分析。
Arch Virol. 2013 Jul;158(7):1477-85. doi: 10.1007/s00705-013-1641-7. Epub 2013 Feb 27.
7
Comparative sequence analysis of major envelope protein gene (B2L) of Indian orf viruses isolated from sheep and goats.从绵羊和山羊中分离出的印度传染性脓疱病毒主要包膜蛋白基因（B2L）的比较序列分析
Vet Microbiol. 2006 Sep 10;116(4):317-24. doi: 10.1016/j.vetmic.2006.04.028. Epub 2006 May 7.
8
Genetic diversity of Orf virus isolated from sheep in Uruguay.从乌拉圭绵羊中分离出的口疮病毒的遗传多样性。
Arch Virol. 2018 May;163(5):1285-1291. doi: 10.1007/s00705-018-3717-x. Epub 2018 Jan 24.
9
Genetic characterization of orf virus associated with an outbreak of severe orf in goats at a farm in Lusaka, Zambia (2015).赞比亚卢萨卡一个农场山羊严重口疮暴发相关口疮病毒的基因特征分析（2015年）
Arch Virol. 2017 Aug;162(8):2363-2367. doi: 10.1007/s00705-017-3352-y. Epub 2017 Apr 4.
10
Isolation and molecular characterization of Orf virus from natural outbreaks in goats of Assam.从阿萨姆邦山羊自然疫情中分离出orf病毒并进行分子特征分析。
Virusdisease. 2015 Jun;26(1-2):82-8. doi: 10.1007/s13337-015-0255-y. Epub 2015 Jun 23.

引用本文的文献

1
A survey of contagious ecthyma and molecular characterization of Orf virus in sheep and goats in Nigeria (2014-2016).尼日利亚绵羊和山羊传染性脓疱病的调查和口疮病毒的分子特征（2014-2016 年）。
Open Vet J. 2022 Jul-Aug;12(4):551-561. doi: 10.5455/OVJ.2022.v12.i4.18. Epub 2022 Aug 17.
2
Genome-Wide Analysis and Molecular Characterization of Orf Virus Strain UPM/HSN-20 Isolated From Goat in Malaysia.从马来西亚山羊中分离出的orf病毒株UPM/HSN-20的全基因组分析及分子特征研究
Front Microbiol. 2022 Jul 11;13:877149. doi: 10.3389/fmicb.2022.877149. eCollection 2022.
3
Molecular detection and characterization of Orf virus from goats in Egypt.

本文引用的文献

1
Field investigation and phylogenetic characterization of orf virus (ORFV) circulating in small ruminants and Pseudocowpoxvirus (PCPV) in dromedary camels of eastern Sudan.苏丹东部小反刍动物中流行的口疮病毒（ORFV）和单峰骆驼中伪牛痘病毒（PCPV）的现场调查及系统发育特征分析
Heliyon. 2020 Mar 21;6(3):e03595. doi: 10.1016/j.heliyon.2020.e03595. eCollection 2020 Mar.
2
Identification of strain diversity and phylogenetic analysis based on two major essential proteins of Orf viruses isolated from several clinical cases reported in Malaysia.基于从马来西亚几例临床病例中分离的 Orf 病毒的两个主要必需蛋白进行的株多样性鉴定和系统进化分析。
Infect Genet Evol. 2020 Jan;77:104076. doi: 10.1016/j.meegid.2019.104076. Epub 2019 Oct 31.
3
从埃及山羊中检测和鉴定口疮病毒。
Open Vet J. 2022 Mar-Apr;12(2):273-280. doi: 10.5455/OVJ.2022.v12.i2.16.
Molecular characterization of Orf virus isolates from Kodai hills, Tamil Nadu, India.
来自印度泰米尔纳德邦科代伊山的口疮病毒分离株的分子特征
Vet World. 2019 Jul;12(7):1022-1027. doi: 10.14202/vetworld.2019.1022-1027. Epub 2019 Jul 12.
4
Comparison of the sensitivity of three cell cultures to ORFV.三种细胞培养物对羊口疮病毒敏感性的比较。
BMC Vet Res. 2019 Jan 7;15(1):13. doi: 10.1186/s12917-018-1760-1.
5
First report of camel contagious ecthyma in Nigeria.尼日利亚骆驼传染性脓疱性皮炎的首次报告。
Open Vet J. 2018;8(2):208-211. doi: 10.4314/ovj.v8i2.16. Epub 2018 Jun 19.
6
Propagation and Molecular Characterization of Bioreactor Adapted Very Virulent Infectious Bursal Disease Virus Isolates of Malaysia.马来西亚生物反应器适应性超强传染性法氏囊病病毒分离株的传播及分子特征分析
J Pathog. 2018 Sep 2;2018:1068758. doi: 10.1155/2018/1068758. eCollection 2018.
7
Dermatopathology of Orf Virus (Malaysian Isolates) in Mice Experimentally Inoculated at Different Sites with and without Dexamethasone Administration.不同部位接种并给予或不给予地塞米松的实验性接种小鼠中羊痘病毒（马来西亚分离株）的皮肤病理学
J Pathog. 2018 Aug 1;2018:9207576. doi: 10.1155/2018/9207576. eCollection 2018.
8
Genetic diversity of Orf virus isolated from sheep in Uruguay.从乌拉圭绵羊中分离出的口疮病毒的遗传多样性。
Arch Virol. 2018 May;163(5):1285-1291. doi: 10.1007/s00705-018-3717-x. Epub 2018 Jan 24.
9
Molecular identification and investigations of contagious ecthyma (Orf virus) in small ruminants, North west Ethiopia.埃塞俄比亚西北部小反刍动物传染性脓疱病（口疮病毒）的分子鉴定与研究
BMC Vet Res. 2018 Jan 15;14(1):13. doi: 10.1186/s12917-018-1339-x.
10
Adaptation and Molecular Characterization of Two Malaysian Very Virulent Infectious Bursal Disease Virus Isolates Adapted in BGM-70 Cell Line.两种在BGM-70细胞系中适应的马来西亚超强毒传染性法氏囊病病毒分离株的适应性及分子特征分析
Adv Virol. 2017;2017:8359047. doi: 10.1155/2017/8359047. Epub 2017 Nov 5.