来自印度泰米尔纳德邦科代伊山的口疮病毒分离株的分子特征
Molecular characterization of Orf virus isolates from Kodai hills, Tamil Nadu, India.
作者信息
Nagarajan G, Pourouchottamane R, Reddy G B Manjunatha, Yogisharadhya R, Sumana K, Rajapandi S, Murali G, Thirumaran S M K, Mallick P K, Rajendiran A S
机构信息
Southern Regional Research Centre, ICAR-Central Sheep and Wool Research Institute, Kodaikanal, Tamil Nadu, India.
ICAR-Central Institute for Research on Goats, Makhdoom, Uttar Pradesh, India.
出版信息
Vet World. 2019 Jul;12(7):1022-1027. doi: 10.14202/vetworld.2019.1022-1027. Epub 2019 Jul 12.
AIM
The present study was carried out to find out the causative agent of exanthematous skin lesions in sheep maintained by Southern Regional Research Centre, Mannavanur, Kodai hills, Tamil Nadu.
MATERIALS AND METHODS
Polymerase chain reaction (PCR) with Orf virus (ORFV) B2L gene-specific primers was carried out by employing the total genomic DNA isolated from the scabs as the template. The ORFV isolates from Kodai hills were characterized by the use of bioinformatics tools.
RESULTS
The amino acid identity of ORFV isolate 1 from Kodai hills is having 98.14%, 96.29%, and 83.59% identity with reference strains of ORFV, virus, and bovine papular stomatitis virus, respectively. Phylogenetic analysis revealed that ORFV isolates from Kodai hills clustered with the other ORFV isolates from different geographical areas of India.
CONCLUSION
The etiological agent of exanthematous skin lesion among sheep of Kodai hills is ORFV.
目的
开展本研究以查明位于泰米尔纳德邦科代山曼纳努尔的南部区域研究中心所饲养绵羊中发疹性皮肤损伤的病原体。
材料与方法
以从痂皮中分离的总基因组DNA为模板，使用奥弗病毒（ORFV）B2L基因特异性引物进行聚合酶链反应（PCR）。利用生物信息学工具对来自科代山的ORFV分离株进行特征分析。
结果
来自科代山的ORFV分离株1与ORFV参考毒株、病毒和牛丘疹性口炎病毒的氨基酸同一性分别为98.14%、96.29%和83.59%。系统发育分析表明，来自科代山的ORFV分离株与来自印度不同地理区域的其他ORFV分离株聚类在一起。
结论
科代山绵羊中发疹性皮肤损伤的病原体是ORFV。
Zotero 插件