埃塞俄比亚西北部小反刍动物传染性脓疱病（口疮病毒）的分子鉴定与研究
Molecular identification and investigations of contagious ecthyma (Orf virus) in small ruminants, North west Ethiopia.
作者信息
Tedla Mebrahtu, Berhan Nega, Molla Wassie, Temesgen Wudu, Alemu Sefinew
机构信息
Department of Biomedical Sciences, University of Gondar, College of Veterinary Medicine and Animal Sciences, P.O. Box: 196, Gondar, Ethiopia.
Department of Biotechnology, University of Gondar, College of Natural and Computational Sciences, P.O. Box: 196, Gondar, Ethiopia.
出版信息
BMC Vet Res. 2018 Jan 15;14(1):13. doi: 10.1186/s12917-018-1339-x.
BACKGROUND
Orf virus, the prototype of parapoxvirus, is the main causative agent of contagious ecthyma. Little is known about the status of the disease in Ethiopia and this study was aimed at determining its status using PCR as a confirmatory tool.
METHODS
a total of 400 randomly selected sheep and goat was screened for the identification of the virus using amplification of B2L gene and transfection of mammalian cells (VERO cells).
RESULTS
Out of 400 animals screened for infection of the virus, 48 animals were found positive to PCR and revealed an overall incidence of 12%. Different epidemiological parameters were considered to look at the association with incidence of the disease and of which, only species of the animal(sheep), non-vaccinated and non-treated animals, nursing animals, poor body condition animals, extensively managed animals, animals having mouth lesion, and study areas having outbreak history showed higher prevalence. A univariate logistic regression analysis showed statistically significant difference in all variables (P < 0.05). Whereas, age and sex of animals showed no significant difference (P < 0.05).
CONCLUSION
The result of the present finding showed high incidence of Orf virus in the region as confirmed through PCR.
背景
羊口疮病毒是副痘病毒的原型，是传染性脓疱性皮炎的主要病原体。埃塞俄比亚该病的发病情况鲜为人知，本研究旨在使用聚合酶链反应（PCR）作为确证工具来确定其发病情况。
方法
共随机选取400只绵羊和山羊，通过扩增B2L基因和转染哺乳动物细胞（VERO细胞）来筛查病毒。
结果
在筛查病毒感染的400只动物中，48只动物PCR检测呈阳性，总体发病率为12%。考虑了不同的流行病学参数以研究其与疾病发病率的关联，其中只有动物种类（绵羊）、未接种疫苗和未治疗的动物、哺乳期动物、身体状况差的动物、粗放管理的动物、有口腔病变的动物以及有疫情爆发历史的研究区域患病率较高。单因素逻辑回归分析显示所有变量均有统计学显著差异（P < 0.05）。而动物的年龄和性别无显著差异（P < 0.05）。
结论
本研究结果表明，经PCR确证该地区羊口疮病毒发病率较高。
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