Department of Analytical Biochemistry, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo, 204-8588, Japan.
Department of Analytical Biochemistry, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo, 204-8588, Japan.
Biochem Biophys Res Commun. 2021 Jul 12;562:100-104. doi: 10.1016/j.bbrc.2021.05.044. Epub 2021 May 26.
Our previous studies have shown that glycerin, which is present at high concentrations in moisturizers and skin lotions, gradually oxidizes to produce methylglyoxal (MGO). In this study, we observed that MGO-treated porcine dermis type-I collagen was carbonylated in an MGO concentration- and time-dependent manner. Furthermore, we examined the structure of advanced glycation end products (AGEs) induced by MGO reacting with type-I collagen. Our findings demonstrate that the α chains of collagen reacted with MGO and easily transformed into a modified protein containing a methylglyoxal-derived hydroimidazolone (MG-H1) moiety in a concentration- and time-dependent manner. Moreover, porcine skin proteins underwent carbonylation when the skin section was treated with MGO for four weeks. Analysis of the structure of AGEs on the carbonylated proteins extracted from MGO-treated skin sections revealed that skin collagen had been converted to MG-H1-modified protein. These novel findings suggest that continuous application of MGO to the skin leads to carbonylation of proteins, which may cause prompt accumulation of MG-H1-modified dermis collagen, thereby resulting in morphological and functional changes of collagen in the skin.
我们之前的研究表明，保湿霜和皮肤乳液中高浓度存在的甘油会逐渐氧化产生甲基乙二醛（MGO）。在这项研究中，我们观察到 MGO 处理的猪真皮 I 型胶原蛋白在 MGO 浓度和时间依赖性方式下发生了羰基化。此外，我们研究了 MGO 与 I 型胶原蛋白反应生成的晚期糖基化终产物（AGEs）的结构。我们的研究结果表明，胶原蛋白的α链与 MGO 反应，并以浓度和时间依赖性的方式轻易转化为含有甲基乙二醛衍生的羟咪唑啉（MG-H1）部分的修饰蛋白。此外，当皮肤切片用 MGO 处理四周时，猪皮蛋白发生了羰基化。对从 MGO 处理的皮肤切片中提取的羰基化蛋白的 AGEs 结构分析表明，皮肤胶原蛋白已转化为 MG-H1 修饰蛋白。这些新发现表明，MGO 持续应用于皮肤会导致蛋白质的羰基化，这可能导致迅速积累 MG-H1 修饰的真皮胶原蛋白，从而导致皮肤胶原蛋白的形态和功能发生变化。
