Egan T M, Saunders N R, Luk S C, Cooper J D
Department of Surgery, University of Toronto, Ontario, Canada.
J Surg Res. 1988 Aug;45(2):204-14. doi: 10.1016/0022-4804(88)90066-2.
Incubation of plasma with zymosan results in complement activation. Infusion of zymosan-activated plasma (ZAP) into the pulmonary circulation in sheep results in a transient rise in pulmonary vascular resistance, accompanied by hypoxemia. This is associated with production of the vasoactive prostaglandin metabolite, thromboxane. We hypothesized that ZAP infusion caused pulmonary edema and may transiently alter pulmonary permeability to protein. Two sets of experiments were conducted involving six sheep. Three sheep underwent open lung biopsies during ZAP infusion, and electron microscopy documented early reversible interstitial pulmonary edema in response to ZAP infusion. Three other sheep were prepared with lung lymph fistulae and subjected to a similar infusion of ZAP. These studies documented a rapid rise in lymph flow and lymph protein clearance, occurring immediately upon infusion of ZAP. These experiments demonstrate a rapid reversible interstitial edema in sheep lung in response to ZAP infusion and suggest that permeability to protein may be transiently altered as a result of this injury.
血浆与酵母聚糖温育会导致补体激活。将酵母聚糖激活的血浆（ZAP）注入绵羊的肺循环会导致肺血管阻力短暂升高，并伴有低氧血症。这与血管活性前列腺素代谢产物血栓素的产生有关。我们推测，注入ZAP会导致肺水肿，并可能短暂改变肺对蛋白质的通透性。进行了两组实验，涉及六只绵羊。三只绵羊在注入ZAP期间接受了开胸肺活检，电子显微镜记录了对注入ZAP的早期可逆性间质性肺水肿。另外三只绵羊制备了肺淋巴瘘，并接受了类似的ZAP注入。这些研究记录了注入ZAP后立即出现的淋巴流量和淋巴蛋白清除率的快速上升。这些实验证明了绵羊肺对注入ZAP的反应中会出现快速可逆的间质性水肿，并表明这种损伤可能会导致蛋白质通透性的短暂改变。
