Egan T M, Saunders N R, Dubois P, Choiniere L, McDonald J W, Cooper J D
Surgery. 1985 Aug;98(2):350-8.
Incubation of plasma with zymosan results in complement activation. Infusion of this "zymosan-activated plasma" (ZAP) into the superior vena cava in sheep results in pulmonary leukostasis, pulmonary hypertension with generation of thromboxane (TXB), and hypoxemia. To examine the contribution of circulating formed elements to TXB generated when ZAP is infused, we studied four pairs of sheep in a cross-circulation model. Acetylsalicylic acid (ASA) irreversibly acetylates cyclooxygenase and blocks TXB production. Four hours after an intravenous dose of ASA (10 mg/kg), no circulating ASA was detectable by high-pressure liquid chromatography. Infusion of ZAP in ASA-treated animals resulted in no pulmonary hypertension and no rise in serum TXB levels. Treated animals were then cross-circulated with untreated sheep through cannulas that attached a carotid artery of each animal to a jugular vein of the other, creating simultaneous arteriovenous fistulas between the animals. A roller pump maintained cross-circulation flow at 450 ml/min for 15 minutes. This resulted in greater than 80% mixing of the circulating blood volumes of each pair of animals, verified by measurement of an intravascular marker. After cross-circulation the ASA-treated animals were still unable to generate TXB in response to ZAP infusion. Untreated animals had a typical response when infused with ZAP after cross-circulation, with elaboration of large amounts of TXB. Since the treated animals with circulating, nonaspirinated formed elements showed no response and the untreated animals with similar blood composition had a normal response, we conclude that circulating formed elements do not contribute significantly to the TXB recovered from sheep infused with activated complement components.
用酵母聚糖孵育血浆会导致补体激活。将这种“酵母聚糖激活血浆”（ZAP）输注到绵羊的上腔静脉中会导致肺白细胞淤滞、伴有血栓素（TXB）生成的肺动脉高压和低氧血症。为了研究在输注ZAP时循环有形成分对TXB生成的作用，我们在交叉循环模型中研究了四对绵羊。乙酰水杨酸（ASA）不可逆地使环氧化酶乙酰化并阻断TXB的产生。静脉注射ASA（10mg/kg）4小时后，通过高压液相色谱法未检测到循环中的ASA。在经ASA处理的动物中输注ZAP不会导致肺动脉高压，血清TXB水平也不会升高。然后将处理过的动物与未处理的绵羊通过套管进行交叉循环，将每只动物的颈动脉与另一只动物的颈静脉相连，在动物之间形成同时的动静脉瘘。一台滚轴泵将交叉循环流量维持在450ml/min，持续15分钟。通过测量血管内标志物证实，这导致每对动物的循环血容量混合超过80%。交叉循环后，经ASA处理的动物在输注ZAP时仍无法产生TXB。交叉循环后，未处理的动物在输注ZAP时出现典型反应，会生成大量TXB。由于具有循环、未被阿司匹林处理的有形成分的处理过的动物没有反应，而具有相似血液成分的未处理动物有正常反应，我们得出结论，循环有形成分对从输注激活补体成分的绵羊中回收的TXB没有显著贡献。
