Reconstruction of Genome-Scale Metabolic Network Model and Nutritional Requirements Analysis of Different Shrimp Commercial Varieties.

As an important tool for systematic analysis, genome-scale metabolic network (GSMN) model has been widely used in various organisms. However, there are few reports on the GSMNs of aquatic crustaceans. is the largest and most productive shrimp species. Feed improvement is one of the important methods to improve the yield of and control water pollution caused by the inadequate absorption of feed. In this work, the first GSMN named GH3005 was reconstructed and applied to the optimization of feed. GH3005 was reconstructed based on the genomic data. The model includes 2,292 reactions and 3,005 genes. GH3005 was used to analyze the nutritional requirements of five different commercial varieties and the genes influencing the metabolism of the nutrients. Based on the simulation, we found that tyrosine-protein kinase src64b like may catalyze different reactions in different commercial varieties. The preference of carbohydrate utilization is different in various commercial varieties, which may due to the different expressions of some genes. In addition, this investigation suggests that a rational and targeted modification in the macronutrient content of shrimp feed would lead to an increase in growth and feed conversion rate. The feed for different commercial varieties should be adjusted accordingly, and possible adjustment schemes were provided. The results of this work provided important information for physiological research and optimization of the components in feed of .