Faculty of Innovative Agriculture and Fishery Establishment Project, Prince of Songkla University, Surat Thani Campus, Surat Thani, 84000, Thailand.
Discipline of Excellence in Sustainable Aquaculture, Prince of Songkla University, Songkhla, 90110, Thailand.
Mol Biol Rep. 2021 May;48(5):4273-4283. doi: 10.1007/s11033-021-06442-6. Epub 2021 May 31.
White scar oyster Crassostrea belcheri is a commercially important bivalve species in Thailand. Appropriate genetic markers are needed for effective management to elevate its production efficiency. Type II microsatellites of C. belcheri were identified and characterized using an Illumina paired-end shotgun sequencing. A total of 14,743,710 reads were generated for which 198,849 reads containing microsatellites and 217,998 microsatellite loci were found. Twenty out of 60 microsatellite loci (33.33%) were polymorphic and these microsatellites were further tested against DNA bulks (N = 10 each) originating from 7 different geographic locations in Thai waters. Results indicated that newly developed microsatellites can be used for genetic diversity analysis of C. belcheri. Genotyping of C. belcheri collected from Surat Thani (Gulf of Thailand; N = 50) were performed. The number of alleles per locus ranged from 2 to 12 (average = 4.95). Observed and expected heterozygosities ranged from 0.0000 to 0.9400 (average = 0.3419) and 0.1139 to 0.8190 (average = 0.5844), respectively. Genome information and 20 newly isolated microsatellites will facilitate further studies in population genetics, stock management, and genetic improvement of C. belcheri in Thailand.
白珍珠蚝（Crassostrea belcheri）是泰国一种具有商业价值的双壳贝类物种。为了提高其生产效率，需要合适的遗传标记进行有效管理。本研究利用 Illumina 配对末端 shotgun 测序技术，鉴定和分析了白珍珠蚝的 II 型微卫星。共生成 14743710 条reads，其中发现了 198849 条含有微卫星的 reads 和 217998 个微卫星位点。在 60 个微卫星位点中，有 20 个（33.33%）是多态的，进一步对来自泰国水域 7 个不同地理区域的 10 个 DNA 块（每个 10 个）进行了这些微卫星的检测。结果表明，新开发的微卫星可用于白珍珠蚝的遗传多样性分析。对来自泰国湾苏拉他尼（Surat Thani）的 50 个白珍珠蚝（C. belcheri）进行了基因分型。每个位点的等位基因数范围为 2-12（平均值为 4.95）。观测和预期杂合度范围分别为 0.0000-0.9400（平均值为 0.3419）和 0.1139-0.8190（平均值为 0.5844）。基因组信息和 20 个新分离的微卫星将有助于进一步研究白珍珠蚝的群体遗传学、种群管理和遗传改良。
