Sato Shuji, Uemoto Yoshinobu, Kikuchi Takashi, Egawa Sachiko, Kohira Kimiko, Saito Tomomi, Sakuma Hironori, Miyashita Satoshi, Arata Shinji, Suzuki Keiichi
National Livestock Breeding Center, Nishigo, Fukushima, Japan.
Miyazaki Branch of National Livestock Breeding Center, Kobayashi, Miyazaki, Japan.
Anim Sci J. 2017 Oct;88(10):1482-1490. doi: 10.1111/asj.12793. Epub 2017 Apr 12.
The aim of the present study was to detect quantitative trait loci affecting fatty acid composition in back fat and intramuscular fat in a Duroc pig population comprising seventh-generation pedigrees using genome-wide association studies (GWAS). In total, 305 animals were genotyped using single nucleotide polymorphisms (SNPs) array and five selected SNPs from regions containing known candidate genes related to fatty acid synthesis or metabolism. In total, 24 genome-wide significant SNP regions were detected in 12 traits, and 76 genome-wide suggestive SNP regions were detected in 33 traits. The Sus scrofa chromosome (SSC) 7 at 10.3 Mb was significantly associated with C17:0 in intramuscular fat, while the SSC9 at 13.6 Mb was significantly associated with C14:0 in intramuscular fat. The SSC12 at 1.0 Mb was significantly associated with C14:0 in back fat and the SSC14 at 121.0 Mb was significantly associated with C18:0 in intramuscular fat. These regions not only replicated previously reported loci containing some candidate genes involved in fatty acid composition (fatty acid synthase and stearoyl-CoA desaturase) but also included several additional related loci.
本研究的目的是利用全基因组关联研究（GWAS），在一个包含第七代系谱的杜洛克猪群体中，检测影响背膘和肌内脂肪中脂肪酸组成的数量性状基因座。总共使用单核苷酸多态性（SNP）阵列对305头动物进行了基因分型，并从与脂肪酸合成或代谢相关的已知候选基因区域中选择了5个SNP。总共在12个性状中检测到24个全基因组显著的SNP区域，在33个性状中检测到76个全基因组提示性SNP区域。位于10.3 Mb的猪7号染色体（SSC）与肌内脂肪中的C17:0显著相关，而位于13.6 Mb的SSC9与肌内脂肪中的C14:0显著相关。位于1.0 Mb的SSC12与背膘中的C14:0显著相关，位于121.0 Mb的SSC14与肌内脂肪中的C18:0显著相关。这些区域不仅复制了先前报道的包含一些参与脂肪酸组成的候选基因（脂肪酸合酶和硬脂酰辅酶A去饱和酶）的基因座，还包括几个额外的相关基因座。
