Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Biomed Chromatogr. 2021 Nov;35(11):e5187. doi: 10.1002/bmc.5187. Epub 2021 Jun 28.
Burdock root is the root of Arctium lappa L., a plant of the Compositae family, which has the effects of dispersing wind and heat, detoxifying and reducing swelling. In order to better control the quality of burdock root, a screening study of quality control indicators was carried out. The current research combines biological activity evaluation with chemical analysis to screen and identify the biologically active compounds of burdock root as chemical components for the quality control of herbal medicine. The efficacy of 10 batches of ethanol extracts of burdock roots was evaluated by a tumor inhibition experiment in S180 tumor-bearing mice. The five main chemical components of these extracts were simultaneously quantitatively measured by ultra-high performance liquid chromatography combined with triple quadrupole mass spectrometry. Pearson correlation analysis was used to establish the relationship between these extracts' biological activity and chemical properties. The results showed that chlorogenic acid, caffeic acid and cynarin were positively correlated with the effect of inhibiting tumor growth, and further bioassays confirmed this conclusion. In conclusion, chlorogenic acid, caffeic acid and cynarin can be used as quality control markers for burdock root's antitumor effect.
牛蒡根是菊科牛蒡属植物牛蒡的根，具有祛风清热、解毒消肿的功效。为了更好地控制牛蒡根的质量，对其质量控制指标进行了筛选研究。本研究将生物活性评价与化学分析相结合，筛选和鉴定牛蒡根的生物活性化合物，作为草药质量控制的化学成分。采用 S180 荷瘤小鼠肿瘤抑制实验评价了 10 批牛蒡根乙醇提取物的功效，同时采用超高效液相色谱-三重四极杆质谱联用技术对这些提取物的 5 种主要化学成分进行了定量测定。采用 Pearson 相关分析建立了这些提取物的生物活性与化学性质之间的关系。结果表明，绿原酸、咖啡酸和菊苣酸与抑制肿瘤生长的效果呈正相关，进一步的生物测定证实了这一结论。综上所述，绿原酸、咖啡酸和菊苣酸可作为牛蒡根抗肿瘤作用的质量控制标志物。
